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An eighth complementation group of rodent cells hypersensitive to ultraviolet radiation

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Somatic Cell and Molecular Genetics

Abstract

Two mutant lines (US31, US46) of mouse lymphoma cells that are hypersensitive to ultraviolet (UV) radiation were previously found to belong to different complementation groups. The mutants were tested for their ability to complement the six known complementation groups of UV-sensitive Chinese hamster ovary (CHO) cells, which are defective in nucleotide excision repair, as well as a seventh group represented by a V79 mutant. Hybrid cells were produced by fusion with polyethylene glycol and tested in situfor UV resistance. The mouse mutant US46 complemented all CHO mutants except UV61. Therefore, US46 is assigned to the same complementation group as UV61, and it is probably defective in the same locus. The mouse mutant US31 produced UV-resistant hybrid cells in each of the seven crosses, indicating that it forms an eighth complementation group among the rodent mutants. Thus, at least eight genes are likely required to repair UV damage in rodent cells.

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Literature cited

  1. Walker, G.C. (1985).Annu. Rev. Biochem. 54:425–457.

    PubMed  Google Scholar 

  2. Walker, G.C. (1985).Annu. Rev. Genet. 19:103–126.

    PubMed  Google Scholar 

  3. Friedberg, E.C. (1987). InMolecular Biology of DNA Repair, (eds.) Collins, A., Johnson, R.T., and Boyle, J. (The Company of Biologists Limited, Cambridge), J. Cell Sci., Suppl. 6, pp. 1–23.

    Google Scholar 

  4. Friedberg, E.C. (1985).DNA Repair (W.H. Freeman, New York).

    Google Scholar 

  5. Cleaver, J.E. (1983). InThe Metabolic Basis of Inherited Disease, 5th ed., (eds.) Stanbury, J.B., Wyngaarden, J.B., Fredrickson, D.S., Goldstein, J.L., Brown, M.S. (McGraw-Hill, New York), pp. 1227–1248.

    Google Scholar 

  6. Fischer, E., Keijzer, W., Thielmann, H.W., Popanda, O., Bohnert, E., Edler, L., Jung, E.G., and Bootsma, D. (1985).Mutat. Res. 145:217–225.

    PubMed  Google Scholar 

  7. Thompson, L.H., Busch, D.B., Brookman, K., Mooney, C.L., and Glaser, D.A. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3734–3737.

    PubMed  Google Scholar 

  8. Thompson, L.H., and Carrano, A.V. (1983). InCellular Responses to DNA Damage, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 11, (eds.) Friedberg, E.C., and Bridges, B.A. (Alan R. Liss, New York), pp. 125–143.

    Google Scholar 

  9. Thompson, L.H., Salazar, E.P., Brookman, K.W., Collins, C.C., Stewart, S.A., Busch, D.B., and Weber, C.A. (1987). InMolecular Biology of DNA Repair, (eds.) Collins, A., Johnson, R.T., and Boyle, J.M. (The Company of Biologists Limited, Cambridge), J. Cell Sci., Suppl. 6, pp. 97–110.

    Google Scholar 

  10. Thompson, L.H., Mitchell, D.L., Regan, J.D., Bouffler, S.D., Stewart, S.A., Carrier, W.L., Nairn, R.S., and Johnson, R.T. (1988).Mutagenesis (in press).

  11. Westerveld, A., Hoeijmakers, J.H.J., van Duin, M., de Wit, J., Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D. (1984).Nature 310:425–429.

    PubMed  Google Scholar 

  12. van Duin, M., de Wit, J., Odijk, H., Westerveld, A., Yasui, A., Koken, M.H.M., Hoeijmakers, J.H.J., and Bootsma, D. (1986).Cell 44:913–923.

    PubMed  Google Scholar 

  13. Weber, C.A., Salazar, E.P., Stewart, S.A., and Thompson, L.H. (1988).Mol. Cell. Biol. 8:1137–1146.

    PubMed  Google Scholar 

  14. Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Somat. Cell Genet. 8:329–345.

    PubMed  Google Scholar 

  15. Thompson, L.H., Carrano, A.V., Sato, K., Salazar, E.P., White, B.F., Stewart, S.A., Minkler, J.L., and Siciliano, J.J. (1987).Somat. Cell Mol. Genet. 13:539–551.

    PubMed  Google Scholar 

  16. Zdzienicka, M.Z., and Simons, J.W.I.M. (1987).Mutat. Res. 178:235–244.

    PubMed  Google Scholar 

  17. Zdzienicka, M.Z., van der Schans, G.P. and Simons, J.W.I.M. (1988).Mutat. Res. 194:165–170.

    PubMed  Google Scholar 

  18. Thompson, L.H., Mooney, C.L., and Brookman, K.W. (1985).Mutat. Res. 150:423–429.

    PubMed  Google Scholar 

  19. Klebe, R.J., and Mancuso, M.G. (1981).Somat. Cell Genet. 7:473–488.

    PubMed  Google Scholar 

  20. Thompson, L.H., Rubin, J.S., Cleaver, J.E., Whitmore, G.F., and Brookman, K. (1980).Somat. Cell Genet. 6:391–405.

    PubMed  Google Scholar 

  21. Sato, L., and Setlow, R.B. (1981).Mutat. Res. 84:443–455.

    PubMed  Google Scholar 

  22. Wade, M.H., and Chu, E.H.Y. (1979).Mutat. Res. 59:49–60.

    PubMed  Google Scholar 

  23. Lehmann, A.R. (1982).Mutat. Res. 106:347–356.

    PubMed  Google Scholar 

  24. Tanaka, K., Kawai, K., Kumahara, Y., Ikenaga, M., and Okada, Y. (1981).Somat. Cell Genet. 7:445–455.

    PubMed  Google Scholar 

  25. Maher, V.M., Ouellette, L.M., Curren, R.D., and McCormick, J.J. (1976).Nature 261:593–595.

    PubMed  Google Scholar 

  26. Myhr, B.C., Turnbull, D., and DiPaolo, J.A. (1979).Mutat. Res. 62:341–353.

    PubMed  Google Scholar 

  27. Stamato, T.D., and Waldren, C.A. (1977).Somat. Cell Genet. 3:431–440.

    PubMed  Google Scholar 

  28. Hoy, C.A., Thompson, L.H., Salazar, E.P., Stewart, S.A. (1985).Somat. Cell Mol. Genet. 11:523–532.

    PubMed  Google Scholar 

  29. Stamato, T.D., Hinkle, L., Collins, A.R.S., and Waldren, C.A. (1977).Somat. Cell Genet. 7:307–320.

    Google Scholar 

  30. Jones, N.J., Cox, R., and Thacker, J. (1987).Mutat. Res. 183:279–286.

    PubMed  Google Scholar 

  31. Jones, N.J., Thompson, L.H., Stewart, S.A., Tucker, J.D., Minkler, J.L., and Carrano, A.V. (1988).J. Cell. Biochem., Suppl. 12A:315.

    Google Scholar 

  32. Thompson, L.H., Brookman, K.W., Weber, C.A., Salazar, E.P., Stewart, S.A., and Carrano, A.V. (1988).J. Cell. Biochem., Suppl. 12A:246.

    Google Scholar 

  33. Thompson, L.H., Weber, C.A., and Carrano, A.V. (1988). InMechanisms and Consequences of DNA Damage Processing, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 83, (ed.) Friedberg, E.C., and Bridges, B.A. (Alan R. Liss, New York), pp. (in press).

    Google Scholar 

  34. Fuller, L.F. and Painter, R.B. (1988).Mutat. Res. 193:109–121.

    PubMed  Google Scholar 

  35. Johnson, R.T., Elliott, G.C., Squires, S., and Joysey, V.C. (1988). (submitted).

  36. Hoy, C.A., Thompson, L.H., Mooney, C.L., and Salazar, E.P. (1985).Cancer Res. 45:1737–1743.

    PubMed  Google Scholar 

  37. Wood, R.D., and Burki, H.J. (1982).Mutat. Res. 95:505–514.

    PubMed  Google Scholar 

  38. Zdzienicka, M.Z., van der Schans, G.P., Westerveld, A., van Zeeland, A.A., and Simons, J.W.I.M. (1988)Mutat. Res. 193:31–41.

    PubMed  Google Scholar 

  39. Robson, C.N., Harris, A.L., and Hickson, I.D. (1985).Cancer Res. 45:5304–5309.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Biomedical Sciences Division, Lawrence Livermore National Laboratory, P.O. Box 5507, 94550, Livermore, California

    L. H. Thompson, E. P. Salazar & S. A. Stewart

  2. Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4-chome, 260, Chiba-shi, Japan

    T. Shiomi

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  1. L. H. Thompson
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  2. T. Shiomi
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  3. E. P. Salazar
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  4. S. A. Stewart
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Thompson, L.H., Shiomi, T., Salazar, E.P. et al. An eighth complementation group of rodent cells hypersensitive to ultraviolet radiation. Somat Cell Mol Genet 14, 605–612 (1988). https://doi.org/10.1007/BF01535314

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  • DOI: https://doi.org/10.1007/BF01535314

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