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Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: A combination of gene transfer and microcell fusion

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Somatic Cell and Molecular Genetics

Abstract

We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven markers (GSR, NP, EST-D, ADK, PEP-S, PGM2, and PEP-B) to hamster chromosome 1. Segregation among the linked markers was induced by X irradiation followed by selection for the retention or loss of human hprt. Cosegregation of markers in independent subclones made it possible to determine the gene order for the seven loci. The gene order proposed for these loci is as follows: pter-GSR-NP-EST-D-ADK-(PEP-S, PGM2)-PEP-B-qter. In addition GSR, NP, EST-D,and ADKhave been assigned to pter-1q12; PEP-Sand PGM2to 1q12–1q21, and PEP-Bto 1q32-1qter. These regional assignments and gene order on chromosome 1 have provided the information relevant to the linkages conserved between Chinese hamster, mouse, and man.

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Author notes

  1. Veena Dhar

    Present address: Department of Human Genetics and Development, Columbia University, 10032, New York, New York

Authors and Affiliations

  1. Department of Microbiology, and Department of Pediatrics, New Jersey Medical School, University of Medicine & Dentistry of New Jersey, 100 Bergen Street, 07103, Newark, New Jersey

    Veena Dhar, Bernard M. Searle & Raghbir S. Athwal

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  1. Veena Dhar
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  2. Bernard M. Searle
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  3. Raghbir S. Athwal
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Dhar, V., Searle, B.M. & Athwal, R.S. Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: A combination of gene transfer and microcell fusion. Somat Cell Mol Genet 10, 547–559 (1984). https://doi.org/10.1007/BF01535220

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  • DOI: https://doi.org/10.1007/BF01535220

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