Skip to main content
SpringerLink
Log in

Characteristics of fatty acid-binding proteins and their relation to mammary-derived growth inhibitor

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Based on sequence relationships the cytoplasmic fatty acid-binding proteins (FABPs) of mammalian origin are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Highly conserved sequences of FABPs within the same type correlate with immunological crossreactivities. Isoforms of hepatic-type FABP are found in several mammalian species and for bovine liver FABP specific shifts in isoelectric points upon lipidation with fatty acids are observed. Isoforms of intestinal-type FABP are not known and the occurrence of cardiac-type isoforms so far is confined to bovine heart tissue. A bovine mammary-derived growth inhibitor (MDGI) is 95% homologous to the cardiac-type FABP from bovine heart. Dissociation constants of FABP/fatty acid complexes are in the range of 1 μM and 1:1 stoichiometries are usually found, but the neutral isoform of hepatic FABP from bovine liver binds 2 fatty acids. On subcellular levels hepatic- and cardiac-type FABPs are differently distributed. Though mainly cytosolic in either case, immunoelectron microscopy as well as a gelchromatographic immunofluorescence assay demonstrate the association of hepatic FABP in liver cells with microsomal and outer mitochondrial membranes and with nuclei, whereas in heart cells cardiac FABP is confined to mitochondria' matrix and nuclei. In mammary epithelial cells MDGI is associated with neither mitochondria nor endoplasmic reticulum, and is expressed in a strictly developmental-dependent spatial and temporal pattern. The specific role proposed for MDGI is to arrest growth of mammary epithelial cells when they become committed to differentiation in the mammary gland.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ockner RK, Manning JA, Poppenhausen RB, Ho WKL: A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium and other tissues. Science 177: 56–58, 1972

    Google Scholar 

  2. Walz DA, Wider MD, Snow GW, Dass C, Desiderio DM: The complete amino acid sequence of porcine gastrotropin, an ileal protein which stimulates gastric acid and pepsinogen secretion. J Biol Chem 263: 14189–14195, 1988

    Google Scholar 

  3. Jones TA, Bergfors T, Sedzik J, Unge T: The three dimensional structure of P2 myelin protein. EMBO J 7: 1597–1604, 1988

    Google Scholar 

  4. Böhmer F-D, Kraft R, Otto A, Wernstedt C, Hellman U, Kurtz A, Müller T, Rohde K, Etzold G, Lehmann W, Langen P, Heldin C-H, Grosse R: Identification of a polypeptide growth inhibitor from bovine mammary gland. Sequence homology to fatty acid- and retinoid-binding proteins. J Biol Chem 262: 15137–15143, 1987

    Google Scholar 

  5. Bordewick U, Heese M, Börchers T, Robenek H, Spener F: Compartmentation of hepatic fatty-acid-binding protein in liver cells and its effect on microsomal phosphatidic acid biosynthesis. Biol Chem Hoppe-Seyler 370: 229–238, 1989

    Google Scholar 

  6. Bass NM: Function and regulation of hepatic and intestinal fatty acid binding proteins. Chem Phys Lipids 38: 95–114, 1985

    Google Scholar 

  7. Meyjohann A, Spener F: Isolation and characterization of the fatty acid binding proteins from bovine small intestine. Abstract P 4, 1st International Workshop on Fatty Acid-Binding Proteins, Maastricht, 1989

  8. Unterberg C, Heidl G, von Bassewitz D-B, Spener F: Isolation and characterization of the fatty acid binding protein from human heart. J Lipid Res 27: 1287–1293, 1986

    Google Scholar 

  9. Börchers T, Unterberg C, Rüdel H, Robenek H, Spener F: Subcellular distribution of cardiac fatty acid-binding protein in bovine heart muscle and quantitation with an enzyme-linked immunosorbent assay. Biochim Biophys Acta 1002: 54–61, 1989

    Google Scholar 

  10. Jagschies G, Reers M, Unterberg C, Spener F: Bovine fatty acid binding proteins. Isolation and characterization of two cardiac fatty acid binding proteins that are distinct from corresponding hepatic proteins. Eur J Biochem 152: 537–545, 1985

    Google Scholar 

  11. Lam KT, Borkan S, Claffey KP, Schwartz JH, Chobanian AV, Brecher P: Properties and differential regulation of two fatty acid binding proteins in the rat kidney. J Biol Chem 263: 15762–15768, 1988

    Google Scholar 

  12. Paulussen RJA, Veerkamp JH: Fatty acid transport in muscle: the role of fatty acid-binding proteins. Biochem Soc Trans 15: 331–336, 1987

    Google Scholar 

  13. Kimura H, Odani S, Suzuki J, Arakawa M, Ono T: Kidney fatty-acid binding protein: identification as α2u,-globulin. FEBS Lett 246: 101–104, 1989

    Google Scholar 

  14. Böhmer F-D, Lehmann W, Noll F, Samtleben R, Langen P, Grosse R: Specific neutralizing antiserum against a polypeptide growth inhibitor for mammary cells purified from bovine mammary gland. Biochim Biophys Acta 846: 145–154, 1985

    Google Scholar 

  15. Billich S, Wissel T, Kratzin H, Hahn U, Hagenhoff B, Lezius AG, Spener F: Cloning of a full-length complementary DNA for fatty-acid-binding protein from bovine heart. Eur J Biochem 175: 549–556, 1988

    Google Scholar 

  16. Bernlohr DA, Angus CM, Lane MD, Bolanowski MK, Kelly TK: Expression of specific mRNAs during adipose differentiation: Identification of a mRNA encoding a homologue of myelin P2 protein. Proc Natl Acad Sci USA 81: 5468–5472, 1984

    Google Scholar 

  17. Haunerland N, Jagschies G, Schulenberg H, Spener F: Fatty-acid-binding proteins. Occurrence of two fatty-acid-binding proteins in bovine liver cytosol and their binding of fatty acids, cholesterol, and other lipophilic ligands. Hoppe-Seyler's Z Physiol Chem 365: 365–376, 1984

    Google Scholar 

  18. Trulzsch D, Arias IM: Z-protein: Isolation and characterization of multiple forms in rat liver cytosol. Arch Biochem Biophys 209: 433–440, 1981

    Google Scholar 

  19. Takahashi K, Odani S, Ono T: Isolation and characterization of the three fractions (DE-I, DE-II and DE-III) of rat-liver Z-protein and the complete primary structure of DE-II. Eur J Biochem 136: 589–601, 1983

    Google Scholar 

  20. Jones PD, Carne A, Bass NM, Grigor MR: Isolation and characterization of fatty acid binding proteins from mammary tissue of lactating rats. Biochem J 251: 919–925, 1988

    Google Scholar 

  21. Börchers T, Hojrup P, Nielsen S, Roepstorff P, Spener F, Knudsen J: Revision of the amino acid sequence of human heart fatty acid binding protein. Mol Cell Biochem, this issue

  22. Offner GD, Brecher P, Sawlivich WB, Costello CE, Troxler RF: Characterization and amino acid sequence of a fatty acid-binding protein from human heart. Biochem J 252: 191–198, 1988

    Google Scholar 

  23. Unterberg C, Börchers T, Hojrup P, Roepstorff P, Knudsen J, Spener F: Cardiac fatty acid binding proteins. Isolation of a mitochondrial fatty acid binding protein and its structural relationship with the cytosolic isoforms. J Biol Chem, in press

  24. Mogensen IB, Schulenberg H, Hansen HO, Spener F, Knudsen J: A novel acyl-CoA binding protein from bovine liver. Effect on fatty acid synthesis. Biochem J 241: 189–192, 1987

    Google Scholar 

  25. Rasmussen JT, Börchers T, Knudsen J: Comparison of the binding affinities of acyl-CoA-binding protein (ACBP) and fatty acid binding protein (FABP) for long-chain acyl-CoA esters. Biochem J 265: 849–855, 1990

    Google Scholar 

  26. Schulenberg-Schell H, Schäfer P, Keuper HJK, Stanislawski B, Hoffmann E, Rüterjans H, Spener F: Interactions of fatty acids with neutral fatty-acid-binding protein from bovine liver. Eur J Biochem 170: 565–574, 1988

    Google Scholar 

  27. Paulussen RJA, van der Logt CPE, Veerkamp JH: Characterization and binding properties of fatty acid-binding proteins from human, pig, and rat heart. Arch Biochem Biophys 264: 533–545, 1988

    Google Scholar 

  28. Lowe JB, Sacchettini JC, Laposata M, McQuillan JJ, Cordon JI: Expression of rat intestinal fatty acid-binding protein in Escherichia coli. Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein. J Biol Chem 262: 5931–5937, 1987

    Google Scholar 

  29. Glatz JFC, Janssen AM, Baerwaldt CCF, Veerkamp JH: Purification and characterization of fatty acid binding proteins from rat heart and liver. Biochim Biophys Acta 837: 57–66, 1985

    Google Scholar 

  30. Keuper HJK, Klein RA, Spener F: Spectroscopic investigations on the binding site of bovine hepatic fatty acid binding protein. Evidence for the existence of a single binding site for two fatty acid molecules. Chem Phys Lipids 38: 159–173, 1985

    Google Scholar 

  31. Sacchettini JC, Cordon JI, Banaszak LJ: The structure of crystalline Escherichia coli-derived rat intestinal fatty acid-binding protein at 2.5 Å resolution. J Biol Chem 263: 5815–5819, 1988

    Google Scholar 

  32. Storch J, Bass NM, Kleinfeld AM: Studies of the fatty acid-binding site of rat liver fatty acid-binding protein using fluorescent fatty acids. J Biol Chem 264: 8708–8713, 1989

    Google Scholar 

  33. Böhmer F-D, Mieth M, Reichmann G, Taube C, Grosse R, Hollenberg MD: A polypeptide growth inhibitor isolated from bovine mammary gland (MDGI) is a lipid carrying protein. J Cell Biochem 38: 199–204, 1988

    Google Scholar 

  34. Behlke J, Mieth M, Böhmer F-D, Grosse R: Hydrodynamic and circular dichroid analysis of mammary derived-growth inhibitor (MDGI). Biochem Biophys Res Commun 161: 363–370, 1989

    Google Scholar 

  35. Fournier NC, Rahim M: Control of energy production in the heart: A new function for fatty acid binding protein. Biochemistry 24: 2387–2396, 1985

    Google Scholar 

  36. Paulussen RJA, Geelen MJH, Beynen AC, Veerkamp JH: Immunochemical quantitation of fatty acid-binding proteins. I. Tissue and intracellular distribution, postnatal development and influence of physiological conditions on rat heart and liver FABP. Biochim Biophys Acta 1001: 201–209, 1989

    Google Scholar 

  37. Müller T, Kurtz A, Vogel F, Breter H, Schneider F, Engström U, Mieth M, Böhmer F-D, Grosse R: A mammary derived growth inhibitor (MDGI) related to 70 kDa antigen identified in nuclei of mammary epithelial cells. J Cell Pysiol 138: 415–423, 1989

    Google Scholar 

  38. Lehmann W, Widmaier R, Langen P: Response of different epithelial cell lines to a mammary derived growth inhibitor (MDGI). Biomed Biochim Acta 48: 143–151, 1989

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Münster, Wilhelm-Klemm-Str. 2, 4400, Münster, Germany

    Friedrich Spener, Christian Unterberg & Torsten Börchers

  2. Central Institute of Molecular Biology, Academy of Sciences of the German Democratic Republic, 1115, Berlin, GDR

    Richard Grosse

Authors
  1. Friedrich Spener
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christian Unterberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Torsten Börchers
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Richard Grosse
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spener, F., Unterberg, C., Börchers, T. et al. Characteristics of fatty acid-binding proteins and their relation to mammary-derived growth inhibitor. Mol Cell Biochem 98, 57–68 (1990). https://doi.org/10.1007/BF00231368

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00231368

Key words

Search

Navigation