Summary
Crude membrane fractions of Dictyostelium discoideum show the capacity to synthesize (1-3H)dolicholphosphate from (1-3H)dolichol. Formation of dolicholphosphate increased continuously over the first 15 min. The reaction rate was nearly linear with respect to the dolichol content up to 150 µM. The phosphate donor for the reaction is CTP. The optimum concentration of CTP is about 0,75 mM. The reaction is dependent on divalent metal ions, magnesium being more effective than calcium or manganese.
The activity of the polyisoprenol kinase depends on the course of the early development. Maximum enzyme activities are present 4–6 h after the induction of the differentiation.
Similar content being viewed by others
References
Elbein, A. D., 1979. Ann. Rev. Plant Physiol. 30: 239–272.
Struck, D. K. and Lennarz, W. J., 1980. The function of saccharide lipids in the synthesis of glycoproteins. In: The Biochemistry of Glycoproteins and Proteoglycans. (Lennarz, W. J., ed.) Plenum Press, New York.
Parodi, A. J. and Leloir, L. F., 1979. Biochim. Biophys. Acta 559: 1–37.
Goldman, R. and Strominger, J. L., 1972. J. Biol. Chem. 247: 5116.
Willoughby, E., Higashi, Y. and Strominger, J. L., 1972. J. Biol. Chem. 247: 5113.
Higashi, Y., Siewert, G. and Strominger, J. L., 1970. J. Biol. Chem. 245: 3683.
Hemming, F. W., 1977. Biochem. Soc. Trans. 5: 1223.
Harford, J. B. and Waechter, C. J., 1980. Biochem. J. 188: 481.
Harford, J. B., Waechter, C. J. and Earl, F. L., 1977. Biochem. Biophys. Res. Commun. 76: 1036.
Rupar, C. A. and Carroll, K. K., 1978. Lipids 13: 291.
Waechter, C. J. and Lennarz, W. J., 1976. Ann. Rev. Biochem. 45: 95.
Rossler, H. H., Peuckert, W., Risse, H.-J. and Eibl, H. J., 1978. Mol. Cell Biochem. 20: 3.
Mills, J. T. and Adamany, A. M., 1978. J. Biol. Chem. 253: 5270.
Allen, C. M., Kalin, J. R., Sack, J. and Verizzo, D., 1978. Biochem. 17: 5020.
Queseda Allue, L. A., 1979. FEBS Lett. 97: 225.
Burton, W. A., Scher, M. G. and Waechter, C. J., 1979. J. Biol. Chem. 254: 7129.
Rip, J. W. and Carroll, K. K., 1980. Can. J. Biochem. 58: 1051.
Coolbear, T. and Mookerjea, S., 1981. J. Biol. Chem. 256: 4529.
Idoyaga-Vargas, V., Belocopitow, E. Mentaberry, A. and Carminatti, H., 1980. FEBS Lett. 112: 63.
Adrian, G. S. and Keenan, R. W., 1979. Biochim. Biophys. Acta 575: 431.
Rip, J. W., Rupar, C. A., Chaudhary, N. and Carroll, K. K., 1981. J. Biol. Chem. 256: 1929.
Rössler, H. H., Schneider-Seelbach, E., Malati, T. and Risse, H.-J., 1981. Mol. Cell. Biochem. 34: 65.
Keenan, R. W. and Kruczek, M., 1975. Anal. Biochem. 69: 504.
Watts, D. J. and Ashworth, J. M., 1970. Biochem. J. 119: 171.
Rössler, H. H. and Risse, H.-J., unpublished data.
Risse, H.-J., Rogge, H., Rath, M. and Platzek, F., 1974. In: Biomembranes, Architecture, Biogenesis, Bioenergetics and Differentiation. (Packer, L., ed.) p. 371. Acad. Press, New York.
Rogge, H., Neises, M. and Risse, H.-J., 1977. Biochim. Biophys. Acta 499: 273.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rössler, H.H., Zimpfer, A. & Risse, HJ. A differentiation-dependend polyisoprenol kinase in Dictyostelium discoideum . Mol Cell Biochem 48, 183–189 (1982). https://doi.org/10.1007/BF00421227
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00421227