Skip to main content
SpringerLink
Log in

Myo-inositol oxygenase from oat seedlings

  • General and Review Articles
  • a. general articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Enzyme preparations from oat seedlings showing the activity ofmyo-inositol oxygenase (E.C.1.13.99.1) have been described previously. In contrast tomyo-inositol oxygenase preparations from other sources, e.g. rat kidney or yeast, the oat enzyme seemed to exhibit a somewhat less stringent activity, acting on other inositols and inositol methyl ethers as well as onmyo-inositol.

By purification of the enzyme present in the extract from oat seedlings with the help of an affinity gel specific for enzymes acting onmyo-inositol a homogeneous enzyme preparation was obtained, which shows the same strict specificity as themyo-inositol oxygenase from other sources. It has a molecular weight of 62,000 and tends to aggregate to oligomers (up to tetramers) under physiological pH-values; in more alkaline media dissociation to monomers is observed. The action on the other inositols and inositol methyl ethers is apparently due to one or more other enzymes, which are also adsorbed on the affinity gel, but can be separated from themyo-inositol oxygenase by elution with increasing concentrations ofmyo-inositol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. M. Gruhner and O. Hoffmann-Ostenhof, Z. physiol. Chem. 347, 278–279 (1966).

    Google Scholar 

  2. K. M. Gruhner and O. Hoffmann-Ostenhof, Monatsh. Chem. 99, 1827–1832 (1968).

    Google Scholar 

  3. F. C. Charalampous and C. Lyras, J. Biol. Chem. 228, 1–13 (1957).

    Google Scholar 

  4. E. Thonet and O. Hoffmann-Ostenhof, Monatsh. Chem. 97, 107–114, 1966.

    Google Scholar 

  5. F. Koller and O. Hoffmann-Ostenhof, Monatsh. Chem. 105, 379–381, 1974.

    Google Scholar 

  6. W. Mejbaum, Z. physiol. Chem. 258, 117–124, 1939.

    Google Scholar 

  7. F. C. Charalampous, J. biol. Chem. 234, 220–227, 1959.

    Google Scholar 

  8. L. J. York, A. P. Grollmann and C. Bublitz, Biochem. biophys. Acta 47, 298–306, 1961.

    Google Scholar 

  9. H. R. Maurer, Disk-Elektrophorese, Walter de Gruyter, Berlin 1968.

    Google Scholar 

  10. H. Stegemann, Z. anal. Chem. 261, 388–391, 1972.

    Google Scholar 

  11. O. Folin and V. Ciocalteu, J. Biol. Chem. 73, 627–650, 1927.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ludwig-Boltzmann-Forschungsstelle für Biochemie, Institut für Allgemeine Biochemie der Universität Wien, Vienna, Austria

    Elisabeth Koller, Franz Koller & O. Hoffmann-Ostenhof

Authors
  1. Elisabeth Koller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Franz Koller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Hoffmann-Ostenhof
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Professor Karl KRATZL on the occasion of his 60th birthday.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koller, E., Koller, F. & Hoffmann-Ostenhof, O. Myo-inositol oxygenase from oat seedlings. Mol Cell Biochem 10, 33–39 (1976). https://doi.org/10.1007/BF01731679

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01731679

Keywords

Search

Navigation