Summary
We have utilized a human tumor clonogenic assay (HTCA), as a disease-oriented drug screening model of new antitumor drugs, to test the antitumor activity of FR66973 and compared the activity with that of its analogous compound, mitomycin C. The overall in vitro response rate (defined as less than 50% survival of tumor colony forming units) for FR66973 against fresh tumor cells obtained from patients with non-small cell lung carcinoma (NSCLC) was 32%, 50% and 89% at 0.1, 1 and 10 μg/ml, respectively, which was superior to that of mitomycin C at the corresponding concentration. Our data suggest that FR66973 is a promising new drug against NSCLC. If phase I toxicities are not prohibitive, FR66973 may also have good activity against NSCLC in clinical phase II trial.
Similar content being viewed by others
References
Kanzawa F, Matsushima Y, Hoshi A, Shimizu E, Saijo N, Miyazawa N: Evaluation of a new drug 7-N-(p-hydroxyphenyl)-mitomycin C [KW2083] against carcinoma of the lung by the human tumor clonogenic assay. Invest New Drugs 3:341–347, 1985
Von Hoff DD, Coltman Jr CA, Forseth B: Activity of mitoxantrone in a human tumor cloning system. Cancer Res 41:1853–1855, 1981
Kiyoto S, Shibata T, Yamashita M, Komori T, Okuhara M, Terano H, Kohsaka M, Aoki H, Imanaka H: A new antitumor antibiotics, FR-900482; (II) Production, isolation, characterization and biological activity. J Antibiot 40:594–599, 1987
Uchida I, Takase S, Kayakiri H, Kiyoto S, Hashimoto M, Tada T, Koda S, Morimoto Y: Structure of FR900482: a novel antitumor antibiotic from a Streptomyces. J Am Chem Soc 109:4108–4109, 1987
Shimomura K, Hirai O, Mizota T, Matsumoto S, Mori J, Shibayama F, Kikuchi H: A new antitumor antibiotic, FR-900482; (III) Antitumor activity in transplantable experimental tumors. J Antibiot 40:600–606, 1987
Kanzawa F, Matsushima Y, Hamburger AW, Ishihara J, Sasaki Y, Shimizu E, Eguchi K, Shinkai T, Saijo N, Miyazawa N: Human tumor clonogenic assay for carcinoma of the lung II. Factors that influence colony formation in soft agar. Oncology 44:150–155, 1987
Alberts DS, Salmon SE, Chen H-SG, Moon TS, Young L, Surwit EA: Pharmacologic studies of anticancer drugs with the human tumor stem cell assay. Cancer Chemother Pharmacol 6:253–264, 1981
Matsushima Y, Kanzawa F, Hoshi A, Shimizu E, Nomori H, Sasaki Y, Saijo N: Time-schedule dependency of the inhibiting activity of various anticancer drugs in the clonogenic assay. Cancer Chemother Pharmacol 14:104–107, 1985
Hirai O, Shimomura K, Mizota T, Matsumoto S, Mori J, Kikuchi H: A new antitumor antibiotic, FR-900482; (IV) Hematological toxicity in mice. J Antibiot 40:607–611, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kanzawa, F., Matsushima, Y., Chiang, CD. et al. A new antitumor antibiotic FR66973: clonogenic in vitro assessment of activity against human non-small cell lung carcinoma. Invest New Drugs 5, 339–343 (1987). https://doi.org/10.1007/BF00169972
Issue Date:
DOI: https://doi.org/10.1007/BF00169972