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Purification of nerve growth factor from the venom of the Central Asian cobraNaja oxiana

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Abstract

A highly purified electrophoreticaly homogeneous protein with a NGF activity of 10·105 BU/mg of protein have been isolated from the venom of the Central Asian cobra by gel-filtration and ion-exchange chromatography followed by preparative isolectric focusing in a thin layer of Sephadex. It has been shown that the NGF isolated is characterized by a molecular weight in the range of 20–30 kD and a pI value of about 7.0.

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Authors
  1. D. Kh. Khamidov
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  2. R. S. Salikhov
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  3. M. G. Khafizova
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  4. L. Ya. Yukel'son
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Additional information

Institute of Biochemistry, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii. No. 4, pp. 546–551, July–August, 1985.

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Khamidov, D.K., Salikhov, R.S., Khafizova, M.G. et al. Purification of nerve growth factor from the venom of the Central Asian cobraNaja oxiana . Chem Nat Compd 21, 512–516 (1985). https://doi.org/10.1007/BF00579148

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