Summary
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1.
Protein IV from synaptosomal fractions ofDrosophila heads is phosphorylatedin vitro by an endogenous cyclic adenosine monophosphate (cAMP)-dependent protein kinase. Thein vivo phosphorylation of this protein is affected by light.
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2.
Two visual mutants,tan andstoned, exhibit altered levels ofin vivo phosphorylation of protein IV. Thetan strain shows depressedin vivo levels of phosphorylation of protein IV, whereasstoned shows an increase in thein vivo level of phosphorylation of this same protein.
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3.
Protein D is phosphorylatedin vitro by an endogenous Ca2+/calmodulindependent protein kinase and has a molecular weight identical to that of protein IV. Thestoned mutant strain shows an increase in thein vivo level of phosphorylation of protein D.
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4.
The data presented here suggest that the phosphorylation of protein IV, and perhaps D, may play a role in the early processing of visual information in the fly.
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References
Albin, E. E., and Newburgh, R. W. (1975). Phosphoprotein phosphatases in the central nervous system ofManduca sexta.Biochim Biophys. Acta 377381–388.
Castellucci, V. F., Kandel, E. R., Schwartz, J. H., Wilson, F. D., Nairn, A. C., and Greengard, P. (1980). Intracellular injection of the catalytic subunit of cAMP-dependent protein kinase simulates facilitation of transmitter release underlying behavioral sensitization inAplysia.Proc. Natl. Acad. Sci. USA 777492–7496.
De Lorenzo, R. J., Freedman, S. D., Yohe, W. B., and Maurer, S. C. (1979). Stimulation of Ca++-dependent neurotransmitter release and presynaptic terminal protein phosphorylation by calmodulin and a calmodulin-like protein isolated from synaptic vesicles.Proc. Natl. Acad. Sci. USA 761838–1842.
Forn, J., and Greengard, P. (1978). Depolarizing agents and cyclic nucleotides regulate the phosphorylation of specific neuronal proteins in rat cerebral cortex slices.Proc. Natl. Acad. Sci. USA 755195–5199.
Greengard, P. (1976). Possible role for cyclic nucleotides and phosphorylated membrane proteins in postsynaptic action of neurotransmitters.Nature 260101–108.
Grigliatti, T. A., Hall, L., Rosenbluth, R., and Suzuki, D. T. (1973). Temperature-sensitive mutations inDrosophila melanogaster. XIV. A selection of immobile adults.Mol. Gen. Genet. 120107–114.
Hotta, Y., and Benzer, S. (1969). Abnormal electroretinograms in visual mutants ofDrosophila.Nature 222354–356.
Huttner, W. B., and Greengard, P. (1979). Multiple phosphorylation sites in protein I and their differential regulation by cAMP and calcium.Proc. Natl. Acad. Sci. USA 765402–5406.
Kelly, L. E. (1981). The regulation of protein phosphorylation in synaptosomal fractions fromDrosophila heads: The role of cyclic adenosine monophosphate and calcium/calmodulin.Comp. Biochem. Physiol. 69861–67.
Kelly, L. E. (1983). An altered electroretinogram transient associated with an unusual jump response in a mutant ofDrosophila.Cell. Mol. Neurobiol. 3143–149.
Klein, M., and Kandel, E. R. (1980). Mechanism of calcium current modulation underlying facilitation and behavioral sensitization inAplysia.Proc. Natl. Acad. Sci. USA 776912–6916.
Klemm, N. (1976). Histochemistry of putative transmitter substances in the insect brain.Prog. Neurobiol. 799–169.
Konopka, R. J. (1972). Abnormal concentrations of dopamine in aDrosophila mutant.Nature 239281–282.
Kuo, J. F., Wyatt, G. R., and Greengard, P. (1971). Cyclic nucleotide-dependent protein kinases. IX Partial purification and some properties of guanosine 3′,5′-monophosphate-dependent and adenosine 3′,5′-monophosphate-dependent protein kinases from various tissues and species of arthropoda.J. Biol. Chem. 2467159–7167.
Lindsley, D., and Grell, E. H. (1967). Genetic variations ofDrosophila melanogaster. Carnegie Institution, Washington, D.C.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193265–275.
Pak, W. L., Grossfield, J., and White, N. V. (1969). Non-phototactic mutants in a study of vision ofDrosophila.Nature 222351–354.
Pellmar, T. C. (1981). Ionic mechanism of a voltage-dependent current elicited by cyclic AMP.Cell. Mol. Neurobiol. 187–97.
Yamanaka, M. K., and Kelly, L. E. (1981). A calcium/calmodulin-dependent cyclic adenosine monophosphate phosphodiesterase fromDrosophila heads.Biochim Biophys. Acta 674277–286.
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Kelly, L.E. The regulation of phosphorylation of a specific protein in synaptosomal fractions fromDrosophila heads: The effects of light and two visual mutants. Cell Mol Neurobiol 3, 127–141 (1983). https://doi.org/10.1007/BF00735277
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DOI: https://doi.org/10.1007/BF00735277