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Genetic and biochemical aspects of sucrase from Drosophila melanogaster

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Abstract

Isoelectrofocusing of abdominal extracts of Drosophila melanogaster revealed the existence of two forms of sucrase (E.C. 3.2.1.26). One form exhibited an isoelectric point of 4.63±0.02 while the other form exhibited an isoelectric point of 4.83±0.02. The localization of the structural gene for sucrase is proposed on the basis of enzyme determinations in a series of duplication- and deletion-bearing aneuploids. We suggest that the sucrase structural gene lies between 31CD and 31EF on the left arm of chromosome 2 and that the two forms of abdominal sucrase derive from a common protein coded for by a single sucrase gene designated Sucr +.

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This work was supported by NRC Grant No. A5860 to J. H. W.

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Oliver, M.J., Williamson, J.H. Genetic and biochemical aspects of sucrase from Drosophila melanogaster . Biochem Genet 17, 897–907 (1979). https://doi.org/10.1007/BF00504311

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