Abstract
The use of L-glutamate dehydrogenase (GLUD) as a reagent in staining mixtures to detect the isozymes of enzymes which catalyze the production of ammonia has been investigated. Methods have been devised for the electrophoresis and detection, using GLUD, of seven enzymes: cytidine deaminase, adenosine deaminase, adenosine monophosphate deaminase, arginase, argininosuccinase, D-amino acid oxidase, and D-aspartate oxidase. GLUD-linked staining methods appear to be sensitive, specific, and of general application.
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This investigation was supported by Public Health Service, Grant No. 1 F22 CA02083-01 of the National Cancer Institute.
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Nelson, R.L., Povey, S., Hopkinson, D.A. et al. Detection after electrophoresis of enzymes involved in ammonia metabolism using L-glutamate dehydrogenase as a linking enzyme. Biochem Genet 15, 1023–1035 (1977). https://doi.org/10.1007/BF00484494
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DOI: https://doi.org/10.1007/BF00484494