Abstract
At the gene locus for liver alcohol dehydrogenase (ADH) of the Japanese quail, three alleles which give electrophoretic variants, A, B, and C, exist. This enzyme is autosomally inherited. Allelic polymorphism was not observed in the chicken, but the wild-type ADH of the chicken can readily be distinguished from A, B, and C of the quail by starch gel electrophoresis. In the development of both species, ADH activity reached a near adult level at about the nineteenth day (a few days after hatching in the quail and a few days before hatching in the chicken). Chicken-quail hybrids at the day of hatching (nineteenth day) revealed the presence of maternally derived quail ADH only, and their ADH activities were about half that of both parental species. Those hybrids which received either A or C allele from the mother quail showed three bands of ADH at the third day after hatching. The chicken and quail alleles began to function in synchronous harmony. One 3-day-old and two adult hybrids which received B allele from the quail, however, still revealed complete absence of the paternally derived chicken ADH.
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Beutler, E., Yeh, M., and Fairbanks, V.F. (1962). The normal human as a mosaic of X-chromosome activity: studies using the gene for G-6-PD deficiency as a marker. Proc. Natl. Acad. Sci. 48 9.
Davidson, R. G., Nitowsky, H. M., and Childs, B. (1963). Demonstration of two populations of cells in the human female heterozygous for glucose-6-phosphate dehydrogenase variants. Proc. Natl. Acad. Sci. 50 481.
Haley, L. E. (1965). Serum albumin polymorphism in quail and chicken-quail hybrids. Genetics 51 983.
Hitzeroth, H., Klose, J., Ohno, S., and Wolf, U. (1968). Asynchronous activation of parental alleles at the tissue-specific gene loci observed on hybrid trout during early development. Biochem. Genet. 1 (3): 287.
Lyon, M. F. (1961). Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature 190 372.
Ohno, S., and Hauschka, T.S. (1960). Allocycly of the X-chromosome in tumors and normal tissues. Cancer Res. 20 541.
Ohno, S. (1966). Cytological and genetic evidence of somatic segregation in mammals, birds and fishes. In Phenotypic Expression, in vitro, Vol. II, Publication of the Tissue Culture Assn., Williams and Wilkins Co., Baltimore.
Pernis, B., Chiappino, G., Kelus, A. S., and Gell, P. G. H. (1965). Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J. Exptl. Med. 122 853.
Shaw, C., and Koen, A. (1964). On the identity of “nothing dehydrogenase.” Histochem. Cytochem. 13 431.
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This work was supported in part by a grant (CA-05138) from the National Cancer Institute, U.S. Public Health Service, and in part by a research fund established in honor of General James H. Doolittle. Contribution No. 20-67, Department of Biology, City of Hope Medical Center.
Dr. Eduardo Castro-Sierra is a fellow of the Institute for Advanced Learning of the City of Hope Medical Center.
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Castro-Sierra, E., Ohno, S. Allelic inhibition at the autosomally inherited gene locus for liver alcohol dehydrogenase in chicken-quail hybrids. Biochem Genet 1, 323–335 (1968). https://doi.org/10.1007/BF00491488
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DOI: https://doi.org/10.1007/BF00491488