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A Simple Method to Calculate Resolving Power and Confidence Interval of QTL Map Location

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Abstract

“Resolving power” is defined as the 95% confidence interval for quantitative trait locus (QTL) map location that would be obtained when scoring an infinite number of markers in a given constellation of a marker-QTL mapping experiment. Resolving power can serve as a close estimate of the confidence interval of QTL map location, as well as a guide to the lower efficient limit of marker spacing in an initial marker-QTL mapping experiment. In the present study, an extensive series of simulations was carried out to provide estimates of resolving power, for backcross (BC) and F2 designs, over a wide range of experimental sizes and of gene effects and dominance at the QTL. From the simulation results, the remarkably simple expressions, 3000/(mNd 2) (where m = 1 for BC and m = 2 for F2; N = population size, and d = allele substitution effect) and 530/Nν (in terms of ν, the proportion of variance explained), were obtained for estimating resolving power. These expressions can provide a convenient guide to planning marker spacing in BC and F2 marker-QTL linkage experiments and for placing confidence intervals about QTL map location obtained in such experiments.

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Authors and Affiliations

  1. Department of Genetics, The Alexander Silberman Life Sciences Institute, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

    A. Darvasi & M. Soller

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  1. A. Darvasi
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  2. M. Soller
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Correspondence to A. Darvasi.

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Darvasi, A., Soller, M. A Simple Method to Calculate Resolving Power and Confidence Interval of QTL Map Location. Behav Genet 27, 125–132 (1997). https://doi.org/10.1023/A:1025685324830

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  • DOI: https://doi.org/10.1023/A:1025685324830

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