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Expression of HIV-1nefin yeast causes membrane perturbation and release of the myristylated Nef protein

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

The human immunodeficiency virus type 1 (HIV-1) Nef protein is essential for AIDS pathogenesis, but its function remains highly controversial. During stresses such as growth in the presence of copper or at elevated temperature, myristylated Nef is released from yeast cells and, after extended culture in stationary phase, it accumulates in the supernatant as a dense membranous material that can be centrifuged into a discrete layer above the cell pellet. This material is unique to Nef-producing cells and represents a convenient source of Nef that may have application in further biological studies. Within the yeast cell, electron microscopic examination shows that Nef localises in novel, membrane-bound bodies. These data support the evidence for a role of Nef in membrane perturbation and suggest that there may be a similar localisation for myristylated Nef in HIV-1 infected cells.

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Authors and Affiliations

  1. CSIRO Molecular Science, Parkville, Vic., Australia

    Jacinta White

  2. Biomolecular Research Institute, 343 Royal Parade, 3052, Parkville, Vic., Australia

    Ian G. Macreadie PhD, Ross Fernley, Laura A. Castelli, Anna Lucantoni & A. Azad

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  1. Ian G. Macreadie PhD
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  2. Ross Fernley
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  3. Laura A. Castelli
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  4. Anna Lucantoni
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  5. Jacinta White
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  6. A. Azad
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Macreadie, I.G., Fernley, R., Castelli, L.A. et al. Expression of HIV-1nefin yeast causes membrane perturbation and release of the myristylated Nef protein. J Biomed Sci 5, 203–210 (1998). https://doi.org/10.1007/BF02253470

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  • DOI: https://doi.org/10.1007/BF02253470

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