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Insulin dominantly suppresses hepatitis B virus gene expression in cultured human hepatoma cells

  • Original Paper
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Journal of Biomedical Science

Abstract

We have shown previously that insulin suppresses the expression of hepatitis B surface antigen (HBsAg) gene from an endogenous integrated viral genome in cultured human hepatoma Hep3B cells. In this study, we demonstrated that insulin suppresses the viral mRNA transcribed from transiently transfected tandem repeat hepatitis B virus (HBV) dimer DNA or DNA fragment that contains only the major HBsAg gene. Insulin treatment also resulted in a decrease in HBV viral particles produced by the HBV-DNA-transfected cells in a dose-dependent manner. Furthermore, when insulin was simultaneously added with glucocorticoid, which stimulates HBV gene expression, the stimulatory effect of glucocorticoid was completely abolished. Our results suggest that insulin has a dominant negative effect on the HBV gene expression in cultured human liver cells.

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Authors and Affiliations

  1. Department of Teaching and Medical Research, Veterans General Hospital, Shih-Pai, Taiperi, Taiwan(ROC)

    Mei-Fang Chen, Hsing-Mei Lin & Chen-Kung Chou MD

  2. Graduate Institute of Genetics and Biochemistry, National Yang-Ming University, Taipei, Taiwan

    Chen-Kung Chou MD

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  1. Mei-Fang Chen
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  2. Hsing-Mei Lin
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  3. Chen-Kung Chou MD
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Chen, MF., Lin, HM. & Chou, CK. Insulin dominantly suppresses hepatitis B virus gene expression in cultured human hepatoma cells. J Biomed Sci 4, 295–299 (1997). https://doi.org/10.1007/BF02258353

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  • DOI: https://doi.org/10.1007/BF02258353

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