Abstract
The time course of changes in the capacity of synchronized yeast cells to be covalently immobilized was determined and interpreted as an indication of chronological changes in the cell's outer-wall proteins. Corroborative evidence was obtained indicating that these transient changes are dependent on protein synthesis and connected with the progression of the cell cycle through the late S and/or early G2 phases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
FarkašV. 1990 Fungal cell walls: their structure, biosynthesis and biotechnological aspects. Acta Biotechnologica 10, 225–238.
JirkůV. 1992. Nystatin and killer toxin sensitivity of free and immobilized Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology 8, 192–195.
JirkůV. & TurkováJ. 1987 Cell immobilization by covalent linkage. Methods in Enzymology 135, 341–357.
KramhøftB., NissenS.B. & ZeuthenE. 1976 The cell cycle in heat- and selection-synchronized Schizosaccharomyces pombe. Carlsberg Research Communications 41, 15–25.
LieblováJ., BeranK. & StreiblováE. 1964 Fractionation of a population of Saccharomyces cerevisiae yeasts by centrifugation in a dextran gradient. Folia Microbiologica 9, 205–213.
MiyataH., MiyataM. & ItoM. 1979 The cell cycle in the fission yeast, Schizosaccharomyces pombe. Cell Structure and Function 4, 81–89.
MüllerR., MumbergD. & LucibelloF.C. 1993 Signals and genes in the control of cell-cycle progression. Biochimica et Biophysica Acta 1155, 151–179.
OlsonB.H. & JohnsonM.J. 1949 Factors producing high yeast yields in synthetic media. Journal of Bacteriology 57, 235–246.
PopoloL., VanoniM. & AlberghinaL. 1982 Control of the yeast cell cycle by protein synthesis. Experimental Cell Reserch 142, 69–78.
VranáD., 1976 Daughter cells as an important factor in determining the physiological state of yeast populations. Biotechnology and Bioengineering 18, 297–309.
VranáD., VotrubaJ. & PlačekJ. 1982 Age-related changes in the physiological state of budding yeast cells. Experimental Cell Research 138, 57–62.
Additional information
The author is with the Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, 166 28 Prague 6, Czech Republic
Rights and permissions
About this article
Cite this article
Jirků, V. Novel way to study the chronological changes in yeast outer-wall proteins. World Journal of Microbiology & Biotechnology 11, 307–309 (1995). https://doi.org/10.1007/BF00367105
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00367105