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The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer

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Abstract

This study was conducted to determine the most suitable method of regeneration by comparing two approaches: transfer of anthers (with and without embryo-like structures) to regeneration conditions after a period of two to four weeks on induction medium (= anther-transfer treatment) and transfer of embryo-like structures to regeneration conditions after five to eight weeks on induction medium. The early transfer of anthers brought about a significant reduction in the number of embryos formed, but nevertheless significantly improved the frequency of plant regeneration. Combining an optimal date of anther transfer with the early addition of colchicine to the induction medium (100 mg l−1 for 1 and 3 days) led to an increase in the number of doubled haploid regenerants. The results indicate that transferring the anthers after 28 days and adding 100 mg l−1 colchicine to the induction medium on one day only caused a significant improvement in the ability of green plants to regenerate (7.0 compared to 0.50) as well as in chromosome doubling (success index: 4.0 compared to 0.33).

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Authors and Affiliations

  1. Department of Biological Sciences, Kuwait University, P.O. Box 5969, Safat, 13060, Kuwait

    A. Redha

  2. Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH) Zurich, Eschikon 33, CH 8315, Lindau, Switzerland

    S.M.S. Islam, B. Büter & P. Stamp

  3. Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH) Zurich, Eschikon 33, CH 8315, Lindau, Switzerland

    J.E. Schmid & J.E. Schmid

Authors
  1. A. Redha
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  2. S.M.S. Islam
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  3. B. Büter
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  4. P. Stamp
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  5. J.E. Schmid
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Correspondence to J.E. Schmid.

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Redha, A., Islam, S., Büter, B. et al. The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer. Plant Cell, Tissue and Organ Culture 63, 167–172 (2000). https://doi.org/10.1023/A:1010708529247

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