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Micropropagation ofPrunus mume

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Abstract

Shoot proliferation from axillary buds ofPrunus mume Sieb. et Zucc. was obtained on Woody Plant Medium (WPM) supplemented with 1 to 5 μM benzyladenine, 3% sorbitol and solidified with 0.5 to 0.7% agar. Effects of different carbon sources on shoot proliferation were examined. Glucose provided better shoot proliferation than sucrose, sorbitol and fructose. In the presence of sucrose, leaf chlorosis occurred and shoots gradually declined. Best rooting percentage was obtained on WPM supplemented with 1 μM naphthaleneacetic acid. Rooted plantlets were acclimatized under intermittent mist. However, survival rate was relatively low (20 to 30%).

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Abbreviations

BA:

benzyladenine

IBA:

indolebutyric acid

MS:

Murashige and Skoog (1962)

NAA:

naphthaleneacetic acid

WPM:

Woody Plant Medium [Lloyd and McCown (1981)]

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Authors and Affiliations

  1. Faculty of Agriculture, Shizuoka University, 422 Ohya, Shizuoka, Japan

    Hisashi Harada & Yasuhiro Murai

Authors
  1. Hisashi Harada
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  2. Yasuhiro Murai
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Harada, H., Murai, Y. Micropropagation ofPrunus mume . Plant Cell Tiss Organ Cult 46, 265–267 (1996). https://doi.org/10.1007/BF02307104

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  • DOI: https://doi.org/10.1007/BF02307104

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