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The effect of acid irrigation on enzyme activities of the single partners of ectomycorrhizas from a limed stand of Norway spruce (Picea abies [L.] Karst.)

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Abstract

The aim of our investigations was to elucidate the effects of acid precipitation on some enzymes of the primary metabolism of ectomycorrhizas. Mycorrhizas of the type of Piceirhiza nigra Gronbach and of Russula ochroleuca (Pers.) Fr. and Tuber puberulum Berk. and Br. were collected from a stand of Norway spruce (Picea abies [L.] Karst.) during the growing seasons of 1991 and 1992. The experimental plots had been limed (Ca: 22 kmol ha-1, Mg: 20 kmol ha-1) in 1984 and exposed to acid irrigation (pH 2.7–2.8, H2SO4: 2 kmol ha-1 a-1) from 1984 to 1990. Crude extracts of mycorrhizas were assayed for the activities of glucose-6-phosphate dehydrogenase (G6P-DH, EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44), NADP-dependent isocitrate dehydrogenase (EC 1.1.1.42) and NAD-dependent glutamate dehydrogenase (EC 1.4.1.2). The influence of the experimental treatments on these enzyme activities of the primary metabolism was generally low. For P. nigra, the activity of G6P-DH was decreased on the irrigated plot (photometric determinations). This seems to be a selective effect on the fungal partner, since quantitative enzyme electrophoresis revealed a decrease in the percentage of the fungal enzyme activity in relation to the total enzyme activity, whereas the content of the fungal compound ergosterol was not affected. A decrease in the fungal G6P-DH activity could also be detected in mycorrhizas of Tuber puberulum. There was also a seasonal variation in the proportion of fungal activity of G6P-DH in relation to the total G6P-DH activity. In the photometric assay (total activity) the effect was not discernible. This is indicative of a degree of regulation between the two partners, which could only be detected by quantitative enzyme electrophoresis. In addition, it could be deduced from the electrophoretograms, that in the case of G6P-DH and 6PG-DH the fungal enzyme activity was dominating in all mycorrhizas studied whereas in the case of ICA-DH the fungal band varied from being conspicuous to absent in different species of mycorrhizas. The banding pattern of G6P-DH was reproducibly different for all investigated species of mycorrhizas.

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Authors and Affiliations

  1. Botanisches Institut, Physiologische Ökologie der Pflanzen, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany

    M. Guttenberger, H. Zick, H. Thelen, T. Wallenda & R. Hampp

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  1. M. Guttenberger
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  2. H. Zick
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  3. H. Thelen
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  4. T. Wallenda
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  5. R. Hampp
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Guttenberger, M., Zick, H., Thelen, H. et al. The effect of acid irrigation on enzyme activities of the single partners of ectomycorrhizas from a limed stand of Norway spruce (Picea abies [L.] Karst.). Plant and Soil 199, 71–81 (1998). https://doi.org/10.1023/A:1017995225968

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