Summary
MPN (most-probable-number) counts of rhizobia by using legumes grown in plastic pouches were essentially equal to pour-plate counts. By using plastic pouches, 60 growth units could be placed in 684 cm2 of bench space, and only 20 minutes were required to prepare and seed 60 plastic pouches for inoculations.
References
Brockwell, J., Applied Microbiol.11, 377–383 (1963).
Cochran, W. G., Biometrics6, 105–116 (1950).
Date, R. A., and Vincent, J. M., Australian J. Exp. Agr. Animal Husb.2, 5–7 (1962).
Finney, D. J., J. Roy. Stat. Soc. Ser. B.9, 46–91 (1947).
Porter, F. E.,et al., Crops Soils18-No. 8, 10–11 (1966).
Purchase, Hilary F., and Nutman, P. S., Ann. Botany London 21, 439–454 (1957).
Sloger, Charles. Plant Physiol.44, 1666–1668 (1969).
Thompson, J. A., and Vincent, J. M., Plant and Soil26, 72–84 (1967).
Tuzimura, K. and Watanabe, I. Soil Sci. Plant Nutr.7, 61–65 (1961).
Wilson, J. K., J. Am. Soc. Agron.18, 911–919 (1926).
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Weaver, R.W., Frederick, L.R. A new technique for most-probable-number counts of Rhizobia. Plant Soil 36, 219–222 (1972). https://doi.org/10.1007/BF01373474
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DOI: https://doi.org/10.1007/BF01373474