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Genetic dissection of cytokinesis

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Abstract

Higher plants have evolved specific mechanisms for partitioning the cytoplasm of dividing cells. In the predominant mode of phragmoplast-assisted cytokinesis, a cell wall and flanking plasma membranes are made de novo from a transient membrane compartment, the cell plate, which in turn forms by vesicle fusion from the centre to the periphery of the dividing cell. Other modes of cytokinesis appear to occur in meiotic cells and developing gametophytes. Here we review recent progress in the analysis of plant cytokinesis, focusing on genetic studies in Arabidopsis which are beginning to identify structural and regulatory components of phragmoplast-assisted cytokinesis. Two classes of mutations have been described. In one class, the defects appear to be confined to cell plate formation, suggesting that the execution of cytokinesis is specifically affected. Mutations in the other class display more general defects in cell division. We also discuss possible roles of proteins that have been localised in cytokinetic cells but not characterised genetically. Finally, mutations affecting meiotic or gametophytic cell divisions suggest that mechanistically different modes of cytokinesis occur in higher plants.

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References

  • Albertsen, M.C. and Palmer, R.G. 1979. A comparative light-and electron-microscopic study of microsporogenesis in male sterile (ms1) and male fertile soybeans (Glycine max (L.) Merr.). Am. J. Bot. 66: 253–265.

    Google Scholar 

  • Amor, Y., Haigler, C.H., Johnson, S., Wainscott, M. and Delmer, D.P. 1995. A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc. Natl. Acad. Sci USA 92: 9353–9357.

    PubMed  Google Scholar 

  • Arioli, T., Peng, L., Betzner, A.S., Burn, J., Wittke, W., Herth, W., Camilleri, C., Hofte, H., Plazinski, J., Birch, R., Cork, A., Glover, J., Redmond, J. and Williamson, R.E. 1998. Molecular analysis of cellulose biosynthesis in Arabidopsis. Science 279: 717–720.

    PubMed  Google Scholar 

  • Asada, T., Kuriyama, R. and Shibaoka, H. 1997. TKRP125, a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic apparatus in tobacco BY-2 cells. J. Cell Sci. 110: 179–189.

    PubMed  Google Scholar 

  • Assaad, F., Mayer, U., Wanner, G. and Jürgens, G. 1996. The KEULE gene is involved in cytokinesis in Arabidopsis. Mol. Gen. Genet. 253: 267–277.

    PubMed  Google Scholar 

  • Beadle, G.W. 1929. A gene for supernumerary mitoses during spore development in Zea mays. Science 70: 406–407.

    Google Scholar 

  • Beadle, G.W. 1932. A gene in Zea mays for failure of cytokinesis during meiosis. Cytologia 3: 142–155.

    Google Scholar 

  • Berger, F. 1999. Endosperm development. Curr. Opin. Plant Biol. 2: 28–32.

    PubMed  Google Scholar 

  • Bögre, L., Calderini, O., Binarova, P., Mattauch, M., Till, S., Kiegerl, S., Jonak, C., Pollaschek, C., Barker, P., Huskisson N.S., Hirt, H. and Heberle-Bors, E. 1999. A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division. Plant Cell 11: 101–113.

    PubMed  Google Scholar 

  • Bonhomme, S., Horlow, C., Vezon, D., de Laissardiere, S., Guyon, A., Ferault, M., Marchand, M., Bechtold, N. and Pelletier, G. 1998. T-DNA mediated disruption of essential gametophytic genes in Arabidopsis is unexpectedly rare and cannot be inferred from segregation distortion alone. Mol. Gen. Genet. 260: 444–452.

    PubMed  Google Scholar 

  • Bowser, J. and Reddy, A.S. 1997. Localization of a kinesin-like calmodulin-binding protein in dividing cells of Arabidopsis and tobacco. Plant J. 12: 1429–1437.

    PubMed  Google Scholar 

  • Brown, R.C., Lemmon, B.E., Nguyen, H. and Olsen, O.A. 1999. Development of endosperm in Arabidopsis thaliana. Sex. Plant Reprod. 12: 32–42.

    Google Scholar 

  • Burgess, R.W., Deitcher, D.L. and Schwarz, T.L. 1997. The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos. J. Cell Biol. 138: 861–875.

    PubMed  Google Scholar 

  • Byers, T.J. and Armstrong, P.B. 1986. Membrane protein redistribution during Xenopus first cleavage. J. Cell Biol. 102: 2176–2184.

    PubMed  Google Scholar 

  • Calderini, O., Bögre, L., Vicente, O., Binarova, P., Heberle-Bors, E. and Wilson, C. 1998. A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells. J. Cell Sci. 111: 3091–3100.

    PubMed  Google Scholar 

  • Chen, Y.C.S. and McCormick, S. 1996. sidecar pollen, an Arabidopsis thaliana male gametophytic mutant with aberrant cell divisions during pollen development. Development 122: 3243–3253.

    PubMed  Google Scholar 

  • Christensen, C.A., Subramanian, S. and Drews, G.N. 1998. Identification of gametophytic mutations affecting female gametophyte development in Arabidopsis. Dev. Biol. 202: 136–151.

    PubMed  Google Scholar 

  • Cleary, A.L. and Smith, L.G. 1998. The Tangled1 gene is required for spatial control of cytoskeletal arrays associated with cell division during maize leaf development. Plant Cell 10: 1875–1888.

    PubMed  Google Scholar 

  • Colasanti, J., Cho, S.O., Wick, S. and Sundaresan, V. 1993. Localization of the functional p34-cdc2 homolog of maize in root tip and stomatal complex cells: association with predicted division sites. Plant Cell 5: 1101–1111.

    PubMed  Google Scholar 

  • Delannay, X. and Palmer, R.G. 1982. Genetics and cytology of the ms4 male-sterile soybean. J. Hered. 73: 219–223.

    Google Scholar 

  • Drews, G.N., Lee, D. and Christensen, C.A. 1998. Genetic analysis of female gametophyte development and function. Plant Cell 10: 5–17.

    PubMed  Google Scholar 

  • Dubois, F., Bui Dang, H.D., Sangwan, R.S. and Durand, J. 1996. The petunia tra1 gene controls cell elongation and plant development, and mediates response to cytokinins. Plant J. 10: 47–59.

    Google Scholar 

  • Eady, C., Lindsey, K., Twell, D. 1995. The significance of microspore division and division symmetry for vegetative cellspecific transcription and generative cell differentiation. Plant Cell 7: 65–74.

    PubMed  Google Scholar 

  • Feiler, H.S., Desprez, T., Santoni, V., Kronenberger, J., Caboche, M. and Traas, J. 1995. The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells. EMBO J. 14: 5626–5637.

    PubMed  Google Scholar 

  • Feldmann, K.A., Coury, D.A. and Christianson, M.L. 1997. Exceptional segregation of a selectable marker (KanR) in Arabidopsis identifies genes important for gametophytic growth and development. Genetics 147: 1411–1422.

    PubMed  Google Scholar 

  • Field, C., Li, R. and Oegema, K. 1999. Cytokinesis in eukaryotes: a mechanistic comparison. Curr. Opin. Cell Biol. 11: 68–80.

    PubMed  Google Scholar 

  • Fineran, B.A., Wild, D.J.C. and Ingerfeld, M. 1982. Initial wall formation in the endosperm of wheat, Triticum aestivum: a reevaluation. Can. J. Bot. 60: 1776–1795.

    Google Scholar 

  • Genschik, P., Criqui M.C., Parmentier, Y., Derevier, A and Fleck, J. 1998. Cell cycle-dependent proteolysis in plants. Identification of the destruction box pathway and metaphase arrest produced by the proteasome inhibitor mg132. Plant Cell 10: 2063–2076.

    PubMed  Google Scholar 

  • Giménez-Abián, M.I., Utrilla, L., Cánovas, J.L., Giménez-Martín, G., Navarrete, M.H., De la Torre, C. 1998. The positional control of mitosis and cytokinesis in higher-plant cells. Planta 204: 37–43.

    PubMed  Google Scholar 

  • Glotzer, M. 1997. The mechanism and control of cytokinesis. Curr. Opin. Cell Biol. 9: 815–823.

    PubMed  Google Scholar 

  • Golubovskaya, I.N. 1989. Meiosis in maize: mei genes and conception of genetic control of meiosis. Adv. Genet. 26: 149–192.

    Google Scholar 

  • Golubovskaya, I.N. and Khristolyubova, N.B. 1985. The cytogenetic evidence of the gene control of meiosis. In: M. Freeling (Ed.) Plant Genetics, Proceedings of the Third Annual ARCO Plant Cell Research Institute, 13–19 April 1985, Keystone, Colorado, USA. A.R. Liss Publishers, New York, pp. 723–738.

    Google Scholar 

  • Gottschalk, W. and Klein, H.D. 1976. The influence of mutated genes on sporogenesis. A survey on the genetic control of meiosis in Pisum sativum. Theor. Appl. Genet. 48: 23–34.

    Google Scholar 

  • Grini, P.E., Schnittger, A., Schwarz, H., Zimmermann, I., Schwab, B., Jürgens, G. and Hülskamp, M. 1999. Isolation of ethyl methanesulfonate-induced gametophytic mutants in Arabidopsis thaliana by a segregation distortion assay using the multimarker chromosome 1. Genetics 151: 849–863.

    PubMed  Google Scholar 

  • Gu, X. and Verma, D.P.S. 1996. Phragmoplastin, a dynamin-like protein associated with cell plate formation in plants. EMBO J. 15: 695–704.

    PubMed  Google Scholar 

  • Gu, X.and Verma, D.P.S. 1997. Dynamics of phragmoplastin in living cells during cell plate formation and uncoupling of cell elongation from the plane of cell division. Plant Cell 9: 157–169.

    PubMed  Google Scholar 

  • Hasezawa, S., Sano, T. and Nagata, T. 1996. Oblique cell plate formation in tobacco BY-2 cells originates in double preprophase bands. J. Plant Res. 107: 355–359.

    Google Scholar 

  • Hauser, B.A., Villanueva, J.M. and Gasser, C.S. 1998. Arabidopsis TSO1 regulates directional processes in cells during floral organogenesis. Genetics 150: 411–423.

    PubMed  Google Scholar 

  • He, C., and Mascarenhas, J.P. 1998. MEI1, an Arabidopsis gene required for male meiosis: isolation and characterization. Sex. Plant Reprod. 11: 199–207.

    Google Scholar 

  • Heese, M., Mayer, U. and Jürgens, G. 1998. Cytokinesis in flowering plants: cellular process and developmental integration. Curr. Opin. Plant Biol. 1: 486–491.

    PubMed  Google Scholar 

  • Howden, R., Park, S.K., Moore, J.M., Orme, J., Grossniklaus, U. and Twell, D. 1998. Selection of T-DNA-tagged male and female gametophytic mutants by segregation distortion in Arabidopsis. Genetics 149: 621–631.

    PubMed  Google Scholar 

  • Huang, B.-Q. and Sheridan, W.F. 1996. Embryo sac development in the maize indeterminate gametophyte1 mutant: abnormal nuclear behavior and defective microtubule organization. Plant Cell 8: 1391–1407.

    PubMed  Google Scholar 

  • Hülskamp, M., Parekh, N.S, Grini, P., Schneitz, K., Zimmermann, I., Lolle, S.J. and Pruitt, R.E. 1997. The STUD gene is required for male-specific cytokinesis after telophase II of meiosis in Arabidopsis thaliana. Dev. Biol. 187: 114–124.

    PubMed  Google Scholar 

  • Hush, J.M., Wu, L., John, P.C.L., Hepler, L.H. and Hepler, P.K. 1996. Plant mitosis promoting factor disassembles the microtubule preprophase band and accelerates prophase progression in Tradescantia. Cell Biol. Int. 20: 275–287.

    PubMed  Google Scholar 

  • Ito, M, Iwase, M., Kodama, H., Lavisse, P., Komamine, A., Nishihama, R., Machida, Y. and Watanabe, A. 1998. A novel cis-acting element in promoters of plant B-type cyclin genes activates M phase-specific transcription. Plant Cell 10: 331–341.

    PubMed  Google Scholar 

  • Jürgens, G. 1996. Cell division and morphogenesis in angiosperm embryogenesis. Sem. Cell Dev. Biol. 7: 867–872.

    Article  Google Scholar 

  • Kakimoto, T. and Shibaoka, H. 1992. Synthesis of polysaccharides in phragmoplasts isolated from tobacco BY2 cells. Plant Cell Physiol. 33: 353–361.

    Google Scholar 

  • Kaul, M.L.H. and Murthy, T.G.K. 1985. Mutant genes affecting higher plant meiosis. Theor. Appl. Genet. 70: 449–466.

    Article  Google Scholar 

  • Kennell, J.C., and Horner, H.T. 1985. Influence of the soybean male-sterile gene (ms1) on the development of the female gametophyte. Can. J. Genet. Cytol. 27: 200–209.

    Google Scholar 

  • Lauber, M.H., Waizenegger, I., Steinmann, T., Schwarz, H., Mayer, U., Hwang, I., Lukowitz, W. and Jürgens, G. 1997. The Arabidopsis KNOLLE protein is a cytokinesis-specific syntaxin. J. Cell Biol. 139: 1485–1493.

    PubMed  Google Scholar 

  • Liu, B., Cyr, R.J. and Palevitz, B.A. 1996. A kinesin-like protein, KatAp, in the cells of Arabidopsis and other plants. Plant Cell 8: 119–132.

    PubMed  Google Scholar 

  • Liu, C.M. and Meinke, D.W. 1998. The titan mutants of Arabidopsis are disrupted in mitosis and cell cycle control during seed development. Plant J. 16: 21–31.

    PubMed  Google Scholar 

  • Liu, C.M., Johnson, S. and Wang, T.L. 1995. cyd, a mutant of pea that alters embryo morphology, is defective in cytokinesis. Dev. Genet. 16: 321–331.

    Google Scholar 

  • Liu, Z., Running, M.P. and Meyerowitz, E.M. 1997. TSO1 functions in cell division during Arabidopsis flower development. Development 124: 665–672.

    PubMed  Google Scholar 

  • Lukowitz, W., Mayer, U. and Jürgens, G. 1996. Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product. Cell 84: 61–71.

    PubMed  Google Scholar 

  • Machida, Y., Nakashima, M., Morikiyo, K., Banno, H., Ishikawa, M., Soyano, T. and Nishihama, R. 1998. MAPKKK-related protein kinase NPK1: regulation of the M phase of plant cell cycle. J. Plant Res. 111: 243–246.

    Google Scholar 

  • Manandhar, G., Apostolakos, P. and Galatis, B. 1996. Cell division of binuclear cells induced by caffeine: spindle organization and determination of division plane. J. Plant Res. 109: 265–275.

    Google Scholar 

  • Mansfield, S.G. and Briarty, L.G. 1990. Endosperm cellularization in Arabidopsis thaliana. Arabidopsis Inf. Serv. 27: 65–72.

    Google Scholar 

  • Mayer, U., Herzog, U., Berger, F., Inzé, D. and Jürgens, G. 1999. Mutations in the PILZ group genes disrupt the microtubule cytoskeleton and uncouple cell cycle progression from cell division in Arabidopsis embryo and endosperm. Eur. J. Cell Biol. 78: 100–108.

    PubMed  Google Scholar 

  • McClinton, R.S. and Sung, Z.R. 1997. Organization of cortical microtubules at the plasma membrane in Arabidopsis. Planta 201: 252–260.

    PubMed  Google Scholar 

  • McCormick, S. 1993. Male gametophyte development. Plant Cell 5: 1265–1275.

    Article  PubMed  Google Scholar 

  • McCoy, T.J. and Smith, L.Y. 1983. Genetics, cytology and crossing behavior of an alfalfa (Medicago sativa) mutant resulting in failure of the postmeiotic cytokinesis. Can. J. Genet. Cytol. 25: 390–397.

    Google Scholar 

  • Mews, M., Sek, F., Moore, R., Volkmann, D., Gunning, B.E.S. and John, P.C.L. 1997. Mitotic cyclin distribution during maize cell division: implications for the sequence diversity and function of cyclins in plants. Protoplasma 200: 128–145.

    Google Scholar 

  • Mitsui, H., Hasezawa, S., Nagata, T. and Takahashi, H. 1996. Cell cycle-dependent accumulation of a kinesin-like protein, KatB/C, in synchronised tobacco BY-2 cells. Plant Mol. Biol. 30: 177–181.

    Article  PubMed  Google Scholar 

  • Moore, J.M., Calzada, J.P., Gagliano, W. and Grossniklaus, U. 1997. Genetic characterization of hadad, a mutant disrupting female gametogenesis in Arabidopsis thaliana. Cold Spring Harb. Symp. Quant. Biol. 62: 35–47.

    PubMed  Google Scholar 

  • Nacry, P., Camilleri, C., Courtial, B., Caboche, M. and Bouchez, D. 1998. Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis. Genetics 149: 641–650.

    PubMed  Google Scholar 

  • Nichols, B.J., Ungermann, C., Pelham, H.R.B., Wickner, W.T. and Haas, A. 1997. Homotypic vacuolar fusion mediated by t-and v-SNAREs. Nature 387: 199–202.

    PubMed  Google Scholar 

  • Nickle, T.C. and Meinke, D.W. 1998. A cytokinesis-defective mutant of Arabidopsis (cyt1) characterized by embryonic lethality, incomplete cell walls, and excessive callose accumulation. Plant J. 15: 321–332.

    PubMed  Google Scholar 

  • Nicol, F., His, I., Jauneau, A., Vernhettes, S., Canut, H. and Hofte, H. 1998. A plasma membrane-bound putative endo-1,4-β-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis. EMBO J. 17: 5563–5576.

    PubMed  Google Scholar 

  • Olsen, O.A., Brown, R.C. and Lemmon, B.E. 1995. Pattern and process of wall formation in developing endosperm. Bioessay 17: 803–812.

    Google Scholar 

  • Owen, H.A. and Makaroff, C.A. 1995. Ultrastructure of microsporogenesis and microgametogenesis in Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija (Brassicaceae). Protoplasma 185: 7–21.

    Google Scholar 

  • Park, J.M., Kang, S.G., Pih, K.T., Jang, H.J., Piao, H.L., Yoon, H.W., Cho, M.J. and Hwang, I. 1997. A dynamin-like protein, ADL1, is present in membranes as a high molecular weight complex in Arabidopsis thaliana. Plant Physiol. 115: 763–771.

    PubMed  Google Scholar 

  • Park, S.K., Howden, R. and Twell, D. 1998. The Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate. Development 125: 3789–3799.

    PubMed  Google Scholar 

  • Patel, S.K., Indig, F.E., Olivieri, N., Levine, N.D. and Latterich, M. 1998. Organelle membrane fusion: a novel function for the syntaxin homolog Ufe1p in ER membrane fusion. Cell 92: 611–620.

    PubMed  Google Scholar 

  • Peirson, B.N., Bowling, S.E. and Makaroff, C.A. 1997. A defect in synapsis causes male sterility in a T-DNA-tagged Arabidopsis thaliana mutant. Plant J. 11: 659–669.

    PubMed  Google Scholar 

  • Peters, J-M. 1998. SCF and APC: the Yin and Yang of cell cycle regulated proteolysis. Curr. Opin. Cell Biol. 10: 759–768.

    PubMed  Google Scholar 

  • Pfeiffer, T.W. and Bingham, E.T. 1983. Abnormal meiosis in alfalfa, Medicago sativa: cytology of 2n egg and 4n pollen formation. Can. J. Genet. Cytol. 25: 107–112.

    Google Scholar 

  • Preuss, D., Rhee, S.Y. and Davis, R.W. 1994. Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes. Science 264: 1458–1460.

    PubMed  Google Scholar 

  • Reynolds, J.O., Eisses, J.F. and Sylvester, A.W. 1998. Balancing division and expansion during maize leaf morphogenesis: analysis of the mutant warty-1. Development 125: 259–268.

    PubMed  Google Scholar 

  • Rothman, J.E. and Warren, G. 1994. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics. Curr. Biol. 4: 220–233.

    PubMed  Google Scholar 

  • Sampson, FB. 1969. Cytokinesis in pollen mother cells of angiosperms, with emphasis on Laurelia novae-zelandiae (Monimiaceae). Cytologia 34: 627–634.

    Google Scholar 

  • Samuels, A.L. and Staehelin, L.A. 1996. Caffeine inhibits cell plate formation by disrupting membrane reorganization just after the vesicle fusion step. Protoplasma 195: 144–155.

    Google Scholar 

  • Samuels, A.L., Giddings, Jr. T.H. and Staehelin, L.A. 1995. Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants. J. Cell Biol. 130: 1345–1357.

    PubMed  Google Scholar 

  • Schneitz, K., Hülskamp, M. and Pruitt, R.E. 1995. Wild-type ovule development in Arabidopsis thaliana: a light microscope study of cleared whole-mount tissue. Plant J. 7: 731–749.

    Google Scholar 

  • Schneitz, K., Hülskamp, M., Kopczak, S.D. and Pruitt, R.E. 1997. Dissection of sexual organ ontogenesis: a genetic analysis of ovule development in Arabidopsis thaliana. Development 124: 1367–1376.

    PubMed  Google Scholar 

  • Scott, R.J., Spielman, M., Bailey, J. and Dickinson, H.G. 1998. Parent-of-origin effects on seed development in Arabidopsis thaliana. Development 125: 3329–3341.

    PubMed  Google Scholar 

  • Shaul, O., Mironov, V., Bursens, S., Van Montagu. M. and Inzé, D. 1996. Two Arabidopsis cyclin promoters mediate distinctive transcriptional oscillation in synchronized tobacco BY-2 cells. Proc. Natl. Acad. Sci USA 93: 4868–4872.

    PubMed  Google Scholar 

  • Sheridan, W.F. and Huang, B-Q. 1997. Nuclear behavior is defective in the maize (Zea mays L.) lethal ovule2 female gametophyte. Plant J 11: 1029–1041.

    Google Scholar 

  • Smith, L.G., Hake, S. and Sylvester, A.W. 1996. The tangled-1 mutation alters cell division orientations throughout maize leaf development without altering leaf shape. Development 122: 481–489.

    PubMed  Google Scholar 

  • Spielman, M., Preuss, D., Li, F.L., Browne, W.E., Scott, R.J. and Dickinson, H.G. 1997. TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. Development 124: 2645–2657.

    PubMed  Google Scholar 

  • Springer, P.S., McCombie, W.R., Sundaresan, V. and Martienssen, R.A. 1995. Gene-trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis. Science 268: 877–880.

    PubMed  Google Scholar 

  • Staehelin, L.A. and Hepler, P.K. 1996. Cytokinesis in higher plants. Cell 84: 821–824.

    Article  PubMed  Google Scholar 

  • Stals, H., Bauwens, S., Traas, J., Van Montagu, M., Engler, G. and Inzé, D. 1997. Plant CDC2 is not only targeted to the pre-prophase band, but also co-localizes with the spindle, phragmoplast, and chromosomes. FEBS Lett. 418: 229–234.

    PubMed  Google Scholar 

  • Sutton, R.B., Fasshauer, D., Jahn, R. and Brunger, A.T. 1998. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution. Nature 395: 347–353.

    Article  PubMed  Google Scholar 

  • Torres Ruiz, R,A. and Jürgens, G. 1994. Mutations in the FASS gene uncouple pattern formation and morphogenesis in Arabidopsis development. Development 120: 2967–2978.

    PubMed  Google Scholar 

  • Traas, J., Bellini, C., Nacry, P., Kronenberger, J., Bouchez, D. and Caboche, M. 1995. Normal differentiation patterns in plants lacking microtubular preprophase bands. Nature 375: 676–677.

    Google Scholar 

  • Twell, D., Park, S.K. and Lalanne, E. 1998. Asymmetric division and cell-fate determination in developing pollen. Trends Plant Sci. 3: 305–310.

    Google Scholar 

  • Ungermann, C., Sato, K. and Wickner, W. 1998. Defining the functions of trans-SNARE pairs. Nature 396: 543–548.

    PubMed  Google Scholar 

  • Valster, A.H. and Hepler, P.K. 1997. Caffeine inhibition of cytokinesis: effect on the phragmoplast cytoskeleton in living Tradescantia stamen hair cells. Protoplasma 196: 155–166.

    Google Scholar 

  • Vaughn, K.C., Hoffman, J.C., Hahn, M.G. and Staehelin, L.A. 1996. The herbicide dichlobenil disrupts cell plate formation: immunogold characterization. Protoplasma 194: 117–132.

    Google Scholar 

  • Verma, D.P.S. and Gu, X. 1996. Vesicle dynamics during cell plate formation in plants. Trends Plant Sci. 1: 145–149.

    Google Scholar 

  • Webb, M.C. and Gunning, B.E.S. 1990. Embryo sac development in Arabidopsis thaliana. I. Megasporogenesis, including the microtubular cytoskeleton. Sex. Plant Reprod. 3: 244–256.

    Google Scholar 

  • Webb, M.C. and Gunning, B.E.S. 1994. Embryo sac development in Arabidopsis thaliana. II. The cytoskeleton during megagametogenesis. Sex. Plant Reprod. 7: 153–163.

    Google Scholar 

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Nacry, P., Mayer, U. & Jürgens, G. Genetic dissection of cytokinesis. Plant Mol Biol 43, 719–733 (2000). https://doi.org/10.1023/A:1006457723760

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