Abstract
We isolated two full-length cDNA clones encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) from potato (Solanum tuberosum) L. tubers. The clones, designated hmg2.2 and hmg3.3, are members of previously described gene subfamilies. In addition to being induced by arachidonic acid in tubers, hmg2.2 transcript accumulates developmentally in young flowers, and in mature sepals and ovaries, whereas transcript for hmg3.3 accumulates in mature petals and anthers. Our data suggest that members of specific HMGR-encoding gene sub-families might be involved in both defense responses and flower development. Accumulation of different HMGR transcripts could provide some control of isoprenoid biosynthesis by producing isoforms specific for classes of end-products produced in particular tissues.
References
Aoyagi K, Beyou A, Moon K, Fang L, Ulrich T: Isolation and characterization of cDNAs encoding wheat 3-hydroxy-3-methylglutaryl coenzyme A reductase. Plant Physiol 102: 623–628 (1993).
Bach TJ, Wettstein A, Boronat A, Ferrer A, Enjuto M, Gruissem W, Narita JO: Properties and molecular cloning of plant HMG-CoA reductase. In: Patterson GW, Nes WD (eds) Physiology and Biochemistry of Sterols, pp. 29–49. American Oil Chemists' Society, Champaign, IL (1991).
Bhattacharyya MK, Paiva NL, Dixon RA, Korth KL, Stermer BA: Features of the hmg1 subfamily of genes encoding HMGCoA reductase in potato Plant Mol Biol 28: 1–15 (1995).
Brooker JD, Russell DW: Properties ofmicrosomal 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pisum sativum seedlings. Arch Biochem Biophys 167: 723–729 (1975).
Caelles C, Ferrer A, Balcells L, Hegardt FG, Boronat A: Isolation and structural characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl coenzyme A reductase. Plant Mol Biol 13: 627–638 (1989).
Chappell J: The biochemistry and molecular biology of isoprenoid metabolism. Plant Physiol 107: 1–6 (1995).
Choi D, Bostock RM, Avdiushko S, Hildebrand DF: Lipidderived signals that discriminate wound-and pathogenresponsive isoprenoid pathways in plants: methyl jasmonate and the fungal elicitor arachidonic acid induce different 3-hydroxy-3-methylglutaryl coenzyme A reductase genes and antimicrobial isoprenoids in Solanum tuberosum L. Proc Natl Acad Sci USA 91: 2329–2333 (1994).
Choi D, Ward BL, Bostock RM: Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid. Plant Cell 4: 1333–1344 (1992).
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159 (1987).
Chye M-L, Tan C-T, Chua N-H: Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed. Plant Mol Biol 19: 473–484 (1992).
Dale S, Arró M, Becerra B, Morrice NG, Boronat A, Hardie DG, Ferrer A: Bacterial expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-CoA reductase (isoform HMGR1) from Arabidopsis thaliana, and its inactivation by phosphorylation at Ser577 by Brassica oleracea 3-hydroxy-3-methylglutaryl-CoA reductase kinase. Eur J Biochem 233: 506–513 (1995).
Fraga BM: Sesquiterpenoids. In: Charlwood BV, Banthorpe DV (eds) Methods in Plant Biochemistry, pp. 145–185. Academic Press, London (1991).
Genschik P, Criqui M-C, Parmentier Y, Marbach J, Durr A, Fleck J, Jamet E: Isolation and chaterization of a cDNA encoding a 3-hydroxy-3-methylglutaryl coenzyme A reductase from Nicotiana sylvestris. Plant Mol Biol 20: 337–341 (1992).
Goldstein JL, Brown MS: Regulation of the mevalonate pathway. Nature 343: 425–430 (1990).
Kuc J: Phytoalexins from the solanaceae. In: Bailey JA, Mans-field JW (eds) Phytoalexins, pp. 81–105. John Wiley, New York (1982).
Maldonado-Mendoza IE, Burnett RJ, Nessler CL: Nucleotide sequence of a cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Catharanthus roseus. Plant Physiol 100: 1613–1614 (1992).
McGarvey DJ, Croteau R: Terpenoid metabolism. Plant Cell 7: 1015-1026 (1995).
Oba K, Kondo K, Doke N, Uritani I: Induction of 3-hydroxy-3-methylglutaryl CoA reductase in potato tubers after slicing, fungal infection or chemical treatment, and some properties of the enzyme. Plant Cell Physiol 26: 873–880 (1985).
Park H, Denbow CJ, Cramer CL: Structure and nucleotide sequence of tomato HMG2 encoding 3-hydroxy-3-methylglutaryl coenzymeAreductase. Plant Mol Biol 20: 327–331 (1992).
Rogers S, Wells R, Rechsteiner M: Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234: 364–368 (1986).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Stermer BA, Bianchini GM, Korth KL: Regulation of HMGCoA reductase activity in plants. J Lipid Res 35: 1133–1140 (1994).
Stermer BA, Bostock RM: Involvement of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the regulation of sesquiterpenoid phytoalexin synthesis in potato. Plant Physiol 84: 404–408 (1987).
Stermer BA, Edwards LA, Edington BV, Dixon RA: Analysis of elicitor-inducible transcripts encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in potato. Physiol Mol Plant Path 39: 135–145 (1991).
Yang Z, Park H, Lacy GH, Cramer CL: Differential activation of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes by wounding and pathogen challenge. Plant Cell 3: 397–405 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Korth, K.L., Stermer, B.A., Bhattacharyya, M.K. et al. HMG-CoA reductase gene families that differentially accumulate transcripts in potato tubers are developmentally expressed in floral tissues. Plant Mol Biol 33, 545–551 (1997). https://doi.org/10.1023/A:1005743011651
Issue Date:
DOI: https://doi.org/10.1023/A:1005743011651