Abstract
In close vicinity of two fus nuclear genes (chloroplast-specific translation elongation factor cEF-G) of soybean (Glycine max) we localized a split nuclear gene coding for a protein with tetratricopeptide repeats (TPR). A full-length cDNA was sequenced (1871 nucleotides). It encodes a protein (569 amino acids) with high sequence identity to the yeast STI1 stress-inducible and the human transformation-sensitive IEF SSP 3521 protein which both carry TPR elements. The soybean gene is heat-inducible. This is the first evidence for the existence of plant genes coding for proteins which belong to the TPR family. We call the gene gmsti and the protein GMSTI in analogy to the yeast counterpart.
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References
Boguski MS, Silorski RS, Hieter P, Goebl M: Expanding family. Nature 346: 114–114 (1990).
Bullock W, Fernandez J, Short J: XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5: 376–378 (1987).
Chou PY, Fasman GD: Predication of protein conformation. Biochemistry 13: 122–145 (1974).
Dean C, van denElzen P, Tamaki S, Dunsmuir P, Bedbrook J: Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family. EMBO J 4. 3055–3061 (1985).
Devereux J, Haeberli P, Smithies O: Comprehensive set of sequence analysis programs for the Vax. Nucl Acids Res 12: 387–395 (1984).
Feinberg AP, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267 (1984).
Goebl M, Yanagida M: A novel protein repeat motif from mitosis to transcription. Trends Biochem Sci 16: 173–177 (1991).
Graham M, Doherty JP, Woodcock DM: Efficient construction of plant genomic libraries requires the use of mrc- host strains and packaging mixes. Plant Mol Biol Rep 8: 18–27 (1990).
Hernández Torres J, Breitenberger C, Spielmann A, Stutz E: Cloning and sequencing of a soybean nuclear gene coding for a chloroplast translation elongation factor EF-G. Biochim Biophys Acta 1174: 191–194 (1993).
Hernández Torres J, Chatellard P, Stutz E: Analysis of nuclear fus genes coding for the chloroplast translation elongation factor cEF-G in soybean. Experientia 50: A24 (1994).
Hirano T, Kinoshita N, Morikawa K, Yanagida M: Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc 2 +. Cell 60: 319–328 (1990).
Honoré B, Leffers H, Madsen P, Rasmussen HH, Vandekerckhove J, Celis JE: Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1. J Biol Chem 267: 8485–8491 (1992).
Nicolet CM, Craig E: Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae. Mol Cell Biol 9: 3638–3646 (1989).
Orrú D, Grosso N, Angeletti B, D'Ambrossio E: An improved protocol to generate high-efficiency single-stranded DNA probes by PCR. Biotechniques 14: 905–906 (1993).
Roberts JK, Key JL: Isolation and characterization of a soybean hsp70 gene. Plant Mol Biol 16: 671–683 (1991).
Sambrook J, Fritsch E, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed.: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson A: DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schori A, Fossati A, Soldati A, Stamp P: Cold tolerance in soybean (Glycine max L. Merr.) in relation to flowering habit, pod set and compensation for lost reproductive organs. Eur J Agron 2: 173–178 (1993).
Sikorski RS, Boguski MS, Goebl M, Hieter P: A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis. Cell 60: 307–317 (1990).
Sikorski RS, Michaud WA, Wootton JC, Boguski MS, Connelly C, Hieter P: TPR proteins as essential components of the yeast cell cycle. Cold Spring Harbor Symp Quant Biol 56: 663–673 (1991).
Sorger PK: Heat shock factor and the heat shock response. Cell 65: 363–366 (1991).
Yost HJ, Lindquist S: RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis. Cell: 185-193 (1986).
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Torres, J.H., Chatellard, P. & Stutz, E. Isolation and characterization of gmsti, a stress-inducible gene from soybean (Glycine max) coding for a protein belonging to the TPR (tetratricopeptide repeats) family. Plant Mol Biol 27, 1221–1226 (1995). https://doi.org/10.1007/BF00020896
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DOI: https://doi.org/10.1007/BF00020896