Abstract
A genomic clone fromHordeum vulgare L. cv. Himalaya contains 700 bp of DNA that is homologous with a high degree of nucleotide sequence similarity to exon 3-intron 3 from the gene for the thiol protease, aleurain. Genomic Southern blot mapping data indicate that this clone in phage λ had not undergone rearrangement, and no other sequences homologous to aleurain are present on it. Although exon 3 in aleurain encodes the polypeptide region cleaved during proteolytic processing of the proenzyme to its mature form, we do not know if the copy is expressed in some other protein. We have mapped the aleurain gene to chromosome 1, and this copy of exon 3-intron 3 to chromosome 2.
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Ganal MW, Bonierbale MW, Roeder MS, Park WD, Tanksley SD: Genetic and physical mapping of the patatin genes in potato and tomato. Mol Gen Genet, in press (1991).
Holwerda BC, Galvin NJ, Baranski TJ, Rogers JC:In vitro processing of aleurain, a barley vacuolar thiol protease. Plant Cell 2: 1091–1106 (1990).
Islam AKMR, Shepherd KW, Sparrow DHB: Isolation and characterization of euplasmic wheat-barley chromosome addition lines. Heredity 46: 161–174 (1981).
Khursheed B, Rogers JC: Barley α-amylase genes: Quantitative comparison of steady-state mRNA levels from individual members of the two different families expressed in aleurone cells. J Biol Chem 263: 18953–18960 (1988).
Rogers JC, Dean D, Heck GR: Aleurain: a barley thiol protease closely related to mammalian cathepsin H. Proc Natl Acad Sci USA 82: 6512–6516 (1985).
Whittier RF, Dean DA, Rogers JC: Sequence analysis of α-amylase and thiol protease genes that are hormonally regulated in barley aleurone cells. Nucl Acids Res 15: 2515–2535 (1987).
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Li, XY., Rogers, S.W. & Rogers, J.C. A copy of exon 3-intron 3 from the barley aleurain gene is present on chromosome 2. Plant Mol Biol 17, 509–512 (1991). https://doi.org/10.1007/BF00040646
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DOI: https://doi.org/10.1007/BF00040646