Abstract
The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae to different concentrations of acids and alkalis was studied in this work. The purpose was to determine their use: (a) in the differentiation of species; (b) for decontamination of clinical materials during the isolation of pathogenic Nocardia. The results showed (1) that both 2 M NH3 and 0.05 M H2SO4 solutions are useful for differentiating species. They can also be used as a complementary method for identification. (2) That both 1 M NH3 and 0.05 M H2SO4 solutions were useful for decontamination of clinical materials during the isolation of pathogenic Nocardia.
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Ajello L, Grant VQ, Gutze MA. The effect of tubercle bacillus concentration procedures on fungi causing pulmonary mycoses. J Lab Clin Med 1951; 38: 486–91.
Murray PR, Heeren RL, Niles C. Effect of Decontamination Procedures on Recovery of Nocardia spp. J Clin Microbiol 1987; 25: 2010–1.
Sommers HM, Good RC. Mycobacterium, p. 290–293. In EH Lennette, A Balows, WJ Hausler (h) and HJ Shadomy (ed), Manual de Microbiología Clínica, ed. 4. American Society for Microbiology, Washington, D.C., 1987.
van Gelderen de Komaid A, Durán EL. The efficiency of 1 M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil. Mycopathologia 1989; 108: 117–23.
van Gelderen de Komaid A, de Laborda R, Salim R. Natural occurrence of Nocardia in soil of Tucumán. Physiological characteristics. Mycopathologia 1987; 99: 15–8.
van Gelderen de Komaid A, de Laborda R. Nocardia en suelo de Tucumán. Fisiología y Patogenicidad de las cepas. II Parte. In Press.
van Gelderen de Komaid A. Physiological and pathogenic characteristics of Nocardia brasiliensis isolated from human mycetomas. Mycopathologia 1989; 105: 111–6.
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van Gelderen de Komaid, A., Durán, E.L. Acid and alkali as aids in differentiation of pathogenic Nocardia and for their isolation from clinical specimens. Mycopathologia 113, 133–137 (1991). https://doi.org/10.1007/BF00436114
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DOI: https://doi.org/10.1007/BF00436114