Abstract
The influence of lymphocytes on the modulation of macrophage functions in altered immune states induced by Cryptococcus neoformans infection in rats has been investigated. In this report we observed a decrease of ‘in vitro’ phagocytic activity by peritoneal cells (PC) from rats that received T suppressor cells induced by cryptococcal infection, against both the same microorganism that stimulated this suppressor population (p<0.05) and another non-pathogenic primary yeast (Candida tropicalis), (p<0.02). The microbicide function of the PC from these animals present a significant decrease in challenge by C. tropicalis (p<0.002) when compared with PC from animals transferred with T normal cells. The transference of T suppressor cells induced by cryptococcal infection in animals immunized with human serum albumin-complete Freund's adjuvant (HSA-CFA) produces a significant alteration of the phagocytosis to HSA-human red cells (HSA-HRC) when compared with the phagocytosis observed in animals that received T normal cells or the phagocytosis of normal animals (p<0.001). We could also observe that the DTH to HSA studied during 30 days was negative in rats transferred with PC sensitizated with HSA and treated with suppressor T cells, when compared with the DTH response of animals transferred with PC-HSA cocultured with normal cells (p<0.05 21st day). The data presented in this paper illustrated that following infection of rats with C. neoformans there is a change in some population of accessory cells behavior reflected by the modification of several functions, such as phagocytosis, lytic activity and antigen presentation.
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References
Blackstock R, McCormack JM, Hall N. Induction of macrophage-suppressive lymphokine by soluble cryptococcal antigens and its association with models of immunologic tolerance. Infection and Immunity 1987; 55: 233–239.
Boyden S. The absorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiproteins sera. Journal of Experimental Medicine 1951; 93: 107–120.
Bradsher RW, Ulmer WC, Marmer DJ, Townsend JW & Jacobs RF. Intracellular growth and phagocytosis of Blastomyces dermatitis by monocyte-derived macrophages from previously infected and normal subjects. Journal of Infections diseases 1985; 151: 57–64.
Brown KM, Kreier SP. Effect of macrophage activation on phagocyte-Plasmodium interaction. Infection and Immunity 1986; 51: 744–749.
Howard DH, Otto U. Experiments on lymphocytemediated cellular immunity in murine histoplasmosis. Infection and Immunity 1977; 16: 226–231.
Julius MH, Simpson E, Herzenberg LA. A rapid method for the isolation of functional thymus-derived murine lymphocytes. European Journal of Immunology 1973; 3: 645–649.
Karaocuri RM, Hall NK, Larsh W. Role of macrophages in immunity and pathogenesis of experimental cryptococcosis induced by the airborne route. II Phagocytosis and intracellular fate of Cryptococcus neoformans. Mykosen 1977; 20: 409–422.
Khusmith S, Druilhe P, Gentilini M. Enhanced Plasmodium falciparum merozoite phagocytosis by monocytes from immune individuals. Infection and Immunity 1982; 35: 874–879.
Lehrer RI. Measurement of candidicidal activity of specific leukocyte types in mixed cell populations. Infection and Immunity 1970; 2: 42–45.
Masih DT, Rubinstein HR, Sotomayor CE, Ferro ME, Riera CM. Non-specific immunosuppression in experimental cryptococcosis in rats. Mycopathologia 1986; 94: 79–84.
Morgan MA, Blackstock RA, Bulmer GS, Hall NK. Modification of macrophage phagocytosis in murine cryptococcosis. Infection and Immunity 1983; 40: 493–500.
Murphy JW, Moorhead JW. Regulation of cell mediated immunity in cryptococcosis. I. Induction of specific afferent T suppressor cells by cryptococcal antigen. Journal of Immunology 1982; 128: 276–283.
Murphy JW, Mosley RL. Regulation of cell mediated immunity in cryptococcosis. III. Characterization of second order T suppressor cells (Ts2). Journal of Immunology 1985; 134: 577–584.
Murphy JW, Mosley RL, Moorhead JW. Regulation of cell mediated immunity in cryptococcosis. II. Characterization of first order T suppressor cells (Ts1) and induction of second order suppressor cells. Journal of Immunology 1983; 130: 2876–2881.
Nickerson DA, Havens RA, Bullock WE. Immunoregulation in disseminated histoplasmosis: characterization of splenic suppressor cells populations. Cellular Immunology 1986; 60: 287–297.
Riera CM, Masih DT, Nobile R. Experimental cryptococcosis in guinea pigs. Mycopathologia 1983; 82: 179–184.
Rogers RS, Balish E. Suppression of lymphocyte blastogenesis by Candida albicans. Clinical Immunology and Immunopathology 1978; 10: 298–305.
Sotomayor CE, Rubinstein HR, Riera CM, Masih DT. Immunosuppression in experimental cryptococcosis in rats. Induction of afferent T suppressor cells to a nonrelated antigen. Journal of Medical and Veterinary Mycology 1987; 25: 67–75.
Unanue ER. Macrophage regulation of immunity (Academic Press, London, 1980).
Unanue ER, Beller DI, Lu CY, Allen PM. Antigen presentation comments on its regulation and mechanism. Journal of Immunology 1984; 132: 1–5.
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Rubinstein, H.R., Sotomayor, C.E., Cervi, L.A. et al. Immunosuppression in experimental cryptococcosis in rats: Modification of macrophage functions by T suppressor cells. Mycopathologia 108, 11–19 (1989). https://doi.org/10.1007/BF00436778
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DOI: https://doi.org/10.1007/BF00436778