Skip to main content
SpringerLink
Log in

Small cytoplasmic RNAs from rat liver mRNP particles

Studies on their structure and function

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Free cytoplasmic 40S mRNP particles from rat liver were treated with EDTA and separated into two populations of RNP particles with sedimentation maxima of 20S and 35S, respectively. A characteristic set of distinct scRNAs is found for 20S and 35S RNP particles.

The sequences of two of the most abundant scRNAs from 20S RNP particles with chain lengths of 104 (α1-RNA) and 124 (β1-RNA) nucleotides, respectively, are presented. α1-RNA shows a high sequence homology to the 3′-end of 18S rRNA. Since α1-RNA carries a cap, it cannot be a degradation product of 18S rRNA. The β1-RNA is strongly post-transcriptionally modified, but uncapped.

When the individual scRNAs of 20S and 35S RNP particles isolated from preparative polyacrylamide gels were assayed for their capability to inhibit in vitro protein synthesis, several potent translational inhibitory RNAs were detected. Particularly, the scRNAs of 147, 203 and 263 nucleotide length associated with the 35S RNP particles turned out to be strong inhibitors of protein synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. WilliamsonR., 1973. FEBS Lett. 37: 1–6.

    Google Scholar 

  2. BrawermanG., 1974. Ann. Rev. Biochem. 43: 635–637.

    Google Scholar 

  3. PreobrazhenskyA. A. & SpirinA. S., 1978. Prog. Nucleic Acid Res. Mol. Biol. 21: 1–38.

    Google Scholar 

  4. MartinT. E., PullmannJ. M. & McMullenM. D., 1980. In: Cell Biology. PrescottD. M. & GoldsteinL. (eds) Vol. 4: pp. 137–174, Academic Press, New York.

    Google Scholar 

  5. CivelliO., VincentA., MaundrellK., BuriJ.-F., & ScherrerK., 1980. Eur. J. Biochem. 107: 577–585.

    Google Scholar 

  6. GoldenbergS. & ScherrerK., 1981. FEBS Lett. 133: 213–216.

    Google Scholar 

  7. LiautardJ. P. & EglyJ. M., 1980. Nucl. Acid. Res. 8: 1793–1804.

    Google Scholar 

  8. SlegersH., DeHerdtE. & KondoM., 1981. Eur. J. Biochem. 117: 111–120.

    Google Scholar 

  9. BagJ. & SellsB. H., 1979. Eur. J. Biochem. 99: 507–516.

    Google Scholar 

  10. CivelliO., VincentA., BuriJ.-F. & ScherrerK., 1976. FEBS Lett. 72: 71–76.

    Google Scholar 

  11. NorthemannW., SchmelzerE. & HeinrichP. C., 1980. Eur. J. Biochem. 112: 451–459.

    Google Scholar 

  12. VincentA., GoldenbergS. & ScherrerK., 1981. Eur. J. Biochem. 114: 179–193.

    Google Scholar 

  13. GrosfeldJ. & LittaverU. U., 1975. Biochem. Biophys. Res. Commun. 67: 176–181.

    Google Scholar 

  14. HeywoodS. M. & KennedyD. S., 1976. Biochemistry 15: 3314–3319.

    Google Scholar 

  15. SlegersH., MettrieR. & KondoM., 1977. FEBS Lett. 30: 390–394.

    Google Scholar 

  16. KühnB., VillringerA., FalkH. & HeinrichP. C., 1982. Eur. J. Biochem. 126: 181–188.

    Google Scholar 

  17. ZieveG. & PenmanS., 1976. Cell 8: 19–31.

    Google Scholar 

  18. PinderJ. C., StaynowD. Z. & GratzerW. B., 1974. Biochemistry 13: 5373–5378.

    Google Scholar 

  19. SchwinghamerM. W. & ShepherdR. J., 1980. Anal. Biochem. 103: 416–434.

    Google Scholar 

  20. WahlG. M., SternM. & StarkG. R., 1979. Proc. Natl. Acad. Sci. USA 76: 3683–3687.

    Google Scholar 

  21. SubrahmanyamC. S., CassidyB., BuschH. & RothblumL. I., 1982. Nucl. Acid. Res. 10: 3667–3682.

    Google Scholar 

  22. TinocoJ.Jr., UhlenbeckO. C. & LevineM. D., 1971. Nature (London) 230: 362–367.

    Google Scholar 

  23. LuomaG. A., BurnsP. D., BruceR. E. & MarshallA. G., 1980. Biochemistry 19: 5456–5462.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemisches Institut, Universität Freiburg, Hermann-Herder-Straße 7, D-7800, Freiburg, FRG

    A. Villringer, B. Kühn, K. Schneider, T. Andus, K. Michels, W. Northemann, G. Krupp, B. Walckhof, H. J. Gross, H. Klump, A. Kroll, Ch. Branlant & P. C. Heinrich

Authors
  1. A. Villringer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Kühn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Andus
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Michels
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. W. Northemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Krupp
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. B. Walckhof
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. H. J. Gross
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. H. Klump
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. A. Kroll
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Ch. Branlant
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. P. C. Heinrich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Villringer, A., Kühn, B., Schneider, K. et al. Small cytoplasmic RNAs from rat liver mRNP particles. Mol Biol Rep 9, 65–74 (1983). https://doi.org/10.1007/BF00777475

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00777475

Keywords

Search

Navigation