Proteasome (prosome) associated endonuclease activity

Abstract

The 20S proteasome (prosome) is a highly organized multiprotein complex with approximate molecular weight of about 700 kDa. Whilst the role of the proteasome in the processing and turnover of cellular proteins is becoming clearer, its relationship with RNA remains still obscure. Here we focus on the nature and function of proteasome associated endonuclease activity. Thus the involvement of a proteasome α-type subunit in RNA-degradation, the catalytic requirements, the interaction of proteasomes with their RNA-substrate and the identification of a well defined cleavage site in the 3-UTR of short-lived cellular mRNAs will be described in detail. All data indicate that proteasomes associated endonuclease activity could be involved in post-transcriptional gene control at the level of translation. Abbreviations: Hu – human; Mu – murine; β IFN – fibroblast interferon; γ IFN – lymphoblast interferon; c FOS – fos proto-oncogene; G-CSF – granulocyte colony stimulating factor.