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The selective iodination of yeast phenylalanine transfer RNA with 125I

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Abstract

Yeast tRNAPhe has been labelled with 125I under conditions which conserve the tertiary structure. Significant labelling was only found to occur on specific cytidines in single stranded regions, while other cytidines in single stranded regions and all those in the double stranded region underwent iodination to a very small extent. The pattern obtained from iodine labelling satisfies the conformation of a model recently proposed for this tRNA.

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Authors and Affiliations

  1. University Chemical Laboratory, Lensfield Road, CB2 1EW, Cambridge, UK

    I. L. Batey & D. M. Brown

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  1. I. L. Batey
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  2. D. M. Brown
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Batey, I.L., Brown, D.M. The selective iodination of yeast phenylalanine transfer RNA with 125I. Molecular Biology Reports 2, 65–72 (1975). https://doi.org/10.1007/BF00357299

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  • DOI: https://doi.org/10.1007/BF00357299

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