Abstract
The optimal magnesium ion concentration for chain initiation in a cell-free system derived from bovine eye lens which synthesizes 4 classes of crystallins appears to be 5 mM. In the synthesis of α-crystallin polypeptides which contain one internal methionine residue and the second one in N-terminal position, Met-RNAfMet functions exclusively as initiator. On the other hand at 5 mM Mg2+ Met-tRNAMet inserts its methionine into the internal position. However, at higher magnesium ion concentrations the initiator tRNA also donates methionine for chain elongation while at the same time the cell-free system loses its capacity to initiate new polypeptides.
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Strous, G.J.A.M., Bloemendal, H. Specificity of lens initiator tRNA for N-terminal recognition. Molecular Biology Reports 1, 99–104 (1973). https://doi.org/10.1007/BF00357588
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DOI: https://doi.org/10.1007/BF00357588