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Preparation of medium for large-scale hybridoma culture

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Journal of tissue culture methods

Summary

Procedures for preparing 400-liter lots of medium for culturing hybridoma cells are detailed. Modifications to Williams essential basal medium are listed and the effectiveness of the medium for culturing hybridoma cells for antibody production is discussed.

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References

  1. Glacken, M.; Fleischaker, R.; Sinskey, A. Mammalian cell culture: Engineering principles and scale-up. Trends in Biotechnology. 1: 102–108; 1983.

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  2. Williams, G. M.; Gunn, J. M. Long-term cell culture of adult rat liver epithelial cells. Expt. Cell Res. 89: 139–142; 1974.

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  3. Lim, F.; Moss, R. Microencapsulation of living cells and tissues. J. Pharm. Sci. 70: 351–354; 1981.

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Authors and Affiliations

  1. Damon Biotech, Inc., 119 4th Avenue, 02194, Needham Heights, Massachusetts, USA

    Randall G. Rupp & Scott D. Geyer

Authors
  1. Randall G. Rupp
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  2. Scott D. Geyer
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Rupp, R.G., Geyer, S.D. Preparation of medium for large-scale hybridoma culture. Journal of Tissue Culture Methods 8, 141–145 (1983). https://doi.org/10.1007/BF01665876

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  • DOI: https://doi.org/10.1007/BF01665876

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