Abstract
Gating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for correct diagnosis. Human lymphocytes are selected by backgating on populations of CD45+high CD14− cells. These reagents are not available widely across species. In veterinary medicine, markers to identify lymphocytes are usually limited to T-lymphocyte, CD4, CD8, and B-lymphocyte surface antigens. A standardized gating technique using a T-lymphocyte antibody is described and is applicable across species where limited phenotype markers are available.
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References
Byrne KM, Kim HW, Chew BP, Reinhart GA, Hayek MG (2000). A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes. Vet Imm Immunopath 73: 167-182.
Calvelli T, Denny TN, Paxton H, Gelman R, Kagan J (1993). Guideline for flow cytometric immunophenotyping: A report from the National Institute of Allergy and Infectious Diseases, Division of AIDS. Cytometry 14: 702-715.
Loken MR, Brosnan JM, Bach BA, Ault KA (1990). Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry 11: 453-459.
Reichert T, DeBruyer M, Deneys V, Totterman T, Lydyard P, Yuksel F, Chapel H, Jewell D, Van hove L, Linden J, Buchner L (1991). Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Imunopathol 60: 190-208.
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Byrne, K.M., Reinhart, G.A. & Hayek, M.G. Standardized flow cytometry gating in veterinary medicine. Methods Cell Sci 22, 191–198 (2000). https://doi.org/10.1023/A:1009896109932
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DOI: https://doi.org/10.1023/A:1009896109932