Summary
The zebrafish is increasingly popular as a nonmammalian model for studies of vertebrate developmental biology, genetics, and toxicology. The availability of cell culture systems makes it possible to address many basic questions using in vitro approaches. Here we describe materials and procedures for initiating cell cultures from zebrafish early (blastula- and gastrula-stage) diploid and haploid embryos and adult tissues (gills, fins, liver, viscera). Zebrafish cells are grown in a complex basal nutrient medium supplemented with insulin, selenite, fetal bovine serum, trout serum, and an extract prepared from rainbow trout embryos. The procedure for preparing trout embryo extract (demonstrated to be mitogenic for a variety of piscine cell lines), is also described.
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Bradford, C.S., Sun, L., Collodi, P. et al. Cell cultures from zebrafish embryos and adult tissues. Journal of Tissue Culture Methods 16, 99–107 (1994). https://doi.org/10.1007/BF01404818
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DOI: https://doi.org/10.1007/BF01404818