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Cloning of primary human tumor colony forming units in glass capillaries

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Journal of tissue culture methods

Summary

Techniques are described for growth of primary human tumor colony-forming units in agar in glass capillary tubes. Tissue recovered from an invasive procedure is made into a single cell suspension. The cells are washed and placed in tissue culture media and agar in glass capillary tubes. After 10 to 14 d of incubation, colonies of tumor cells are visible in the capillaries. This technique can be used to determine the sensitivity pattern of patients' tumors to conventional and investigational anticancer agents. The technique can also be used to screen for antitumor activity of new chemical entities.

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Authors and Affiliations

  1. Cancer Therapy and Research Center of South Texas, 4450 Medical Drive, 78229, San Antonio, Texas, USA

    Daniel D. Von Hoff, Marty Marshall, Donna Chacon, Belinda Leal, Frank Rodriguez, Sonya Drabek, Robert Garcia & Barbara Brown

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  1. Daniel D. Von Hoff
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Von Hoff, D.D., Marshall, M., Chacon, D. et al. Cloning of primary human tumor colony forming units in glass capillaries. Journal of Tissue Culture Methods 13, 125–131 (1991). https://doi.org/10.1007/BF01666143

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