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Shortening properties of two biochemically defined muscle fibre types of the Norway lobster Nephrops norvegicus L.

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Abstract

Mechanical properties of myofibrillar bundles from single chemically skinned fibres from the superficial abdominal flexor muscle of the Norway lobster Nephrops norvegicus were measured, and the protein content of these fibres was analysed by SDS-PAGE. Two slow fibre phenotypes (S1, S2) were distinguished on the basis of their myofibrillar protein assemblages. Data from 9 S1 and 8 S2 fibres obtained at similar sarcomere length demonstrate significant differences between the fibre types in maximal tension (N cm-2, S1: 10.5 ± 3.9; S2: 3.1 ± 0.8), in the delay of the peak of stretch activation (ms, S1: 122 ± 18; S2: 412 ± 202), in fibre stiffness (N cm-2 per nm half sarcomere, S1: 0.36 ± 0.19; S2: 0.09 ± 0.03) and in maximal shortening velocity (fibre length s-1, S1: 0.53 ± 0.10; S2: 0.27 ± 0.06). Furthermore, the maximal power output of the type S1 fibres was about five times larger than that of S2 fibres. The power output was maximal at lower loads in S1 fibres (relative load = 0.37 ± 0.04) than in S2 fibres (relative load = 0.44 ± 0.05). This study represents a comprehensive investigation of two slow muscle fibre types which are thought to be specialized for slow movements (S1 fibres) and for the postural control of the abdomen (S2 fibres).

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Authors and Affiliations

  1. Division of Environmental and Evolutionary Biology, University of Glasgow, Graham Kerr Building, Glasgow, G12 8QQ, Scotland, UK

    Janet M. Holmes & Douglas M. Neil

  2. Institut für Zoologie, Universität Salzburg, Hellbrunnerstraße 34, A-5020, Salzburg, Austria

    Karlheinz Hilber & Stefan Galler

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  1. Janet M. Holmes
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  2. Karlheinz Hilber
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  3. Stefan Galler
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  4. Douglas M. Neil
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Holmes, J.M., Hilber, K., Galler, S. et al. Shortening properties of two biochemically defined muscle fibre types of the Norway lobster Nephrops norvegicus L.. J Muscle Res Cell Motil 20, 265–278 (1999). https://doi.org/10.1023/A:1005481725344

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