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Thermodynamics of a 24-Mer Triple Helix Formation and Stability

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Abstract

In this work we report a thermodynamic characterization of stability and melting behaviour of two 24-mer DNA triplexes. The third strand, that binds the Watson-Crick double helix with Hoogsteen hydrogen bonds, contains 3′-3′ phosphodiester junction that determines the polarity inversion. The target double helix is composed of adjacent and alternate fragments of oligopurine-oligopyrimidine tracts. The two helices differ from the substitution of the cytosine, involved in the junction, with the thymine. Calorimetric data reported here provide a quantitative measure of the influence of pH and base modification on the stability of a DNA triplex.

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Authors and Affiliations

  1. Department of Chemistry, University 'Federico II' of Naples, Via Mezzocannone 4, 80134, Naples

    C. Giancola, A. Buono & G. Barone

  2. Department of Organic Chemistry and Biochemistry, University 'Federico II' of Naples, Via Mezzocannone 16, 80134, Naples, Italy

    L. De Napoli, D. Montesarchio, D. Palomba & G. Piccialli

Authors
  1. C. Giancola
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  2. A. Buono
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  3. G. Barone
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  4. L. De Napoli
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  5. D. Montesarchio
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  6. D. Palomba
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  7. G. Piccialli
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Giancola, C., Buono, A., Barone, G. et al. Thermodynamics of a 24-Mer Triple Helix Formation and Stability. Journal of Thermal Analysis and Calorimetry 56, 1177–1184 (1999). https://doi.org/10.1023/A:1010117431041

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  • DOI: https://doi.org/10.1023/A:1010117431041

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