Abstract
A radioimmunoassay procedure to measure estradiol levels in human serum was developed using125I labeled estradiol and antiestradiol antibody raised in-house using the estradiol-6-CMO-BSA as the immunogen. Controlled process serum was used to maintain the serum matrix and sample-standard identity. The assay was validated by analysing several (n=40) samples and comparing with established commercial assays. The assay had a sensitivity of 35 pg/mL and a range of 50 pg/mL to 5 ng/mL. The interassay C.V. were between 2 to 10% while the intraassay C.V. were between 2 and 8%. This assay is sensitive enough for monitoring ovulation and hypersecretions in ovarian tumours and was primarily developed to be used as an in-house assay in the local hospitals.
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Korde, A., Venkatesh, M., Balakrishnan, S.A. et al. A radioimmunoassay for serum estradiol. Journal of Radioanalytical and Nuclear Chemistry, Articles 206, 219–226 (1996). https://doi.org/10.1007/BF02039646
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DOI: https://doi.org/10.1007/BF02039646