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Studies on carrageenans and effects of seawater phosphorus concentration on carrageenan content and growth ofAgardhiella subulata (C. Agardh) Kraft and Wynne (Rhodophyceae, Solieriaceae)

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Abstract

Gas liquid chromatography, chemical analyses, and infrared and13C-NMR spectroscopies indicated that phycocolloids extracted fromAgardhiella subulata had a dominant ι-carrageenan feature with less deviant ι-carrageenan and υ-carrageenan. The presence of methylated galactose and a small contamination by xylose were registered. Unattached plants were cultivated for 4 weeks in tanks receiving seawater enriched with 53.5 µM nitrate and 0 to 20 µM phosphate (Pi) week−1. The growth was phosphorus (P)-limited up to a tissue P content of 0.14 ± 0.03% dry weight. Maximal specific growth rate and carrageenan content were observed with enrichments of 6 µM Pi and 3 µM Pi, respectively. Hence carrageenan production was promoted in the range of 3–6 µM Pi. Further Pi enrichment was useless. This phenomenon, observed with P nutrition, is comparable to the ‘Neish effect’ in nitrogen nutrition studies.

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  1. T. Chopin

    Present address: Biology Department, University of New Brunswick, P.O. Box 5050, E2L 4L5, Saint John, N.B., Canada

Authors and Affiliations

  1. Harbor Branch Oceanographic Inst., 34946, Fort Pierce, Florida, USA

    T. Chopin, M. D. Hanisak & F. E. Koehn

  2. EPPV, Univ. Lille Flandres Artois, 59655, Villeneuve d'Ascq Cedex, France

    T. Chopin & J. Mollion

  3. INSERM-U42, 59650, Villeneuve d'Ascq, France

    S. Moreau

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Presented in part at the XIIIth International Seaweed Symposium, University of British Columbia, Vancouver, Canada, August 1989.

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Chopin, T., Hanisak, M.D., Koehn, F.E. et al. Studies on carrageenans and effects of seawater phosphorus concentration on carrageenan content and growth ofAgardhiella subulata (C. Agardh) Kraft and Wynne (Rhodophyceae, Solieriaceae). J Appl Phycol 2, 3–16 (1990). https://doi.org/10.1007/BF02179764

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