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Comparison of ITS RFLP patterns of Gracilaria (Rhodophyceae, Gracilariales) populations from Chile and New Zealand and an examination of interfertility of Chilean morphotypes

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Abstract

Restriction fragment length polymorphism (RFLP) patterns of the internal transcribed spacer (ITS) of the nuclear ribosomal cistron and crossability trials were used to characterize four morphotypes of Gracilaria from Lenga, Isla Santa María and Maullín, Chile, and two morphotypes from sites in New Zealand. PCR products from all Chilean morphotypes resulted in a major single band of ca. 1198 bp. ITS-RFLP profiles generated with the restriction enzymes Cla I, Hae III, Pst I, Hha I, Rsa I and Taq I, were identical in all cases. All crosses within, as well as between, morphotypes resulted in cystocarp differentiation, with the production of viable carpospores. Based upon these data, it is concluded that the four morphotypes from Chile correspond to a single species, G. chilensis, and that the ITS-RFLP pattern is a useful marker to predict genetic relatedness at the specific level in Gracilaria. A comparison of the ITS-RFLP patterns of the Chilean morphotypes with the patterns of two samples of G. chilensis from New Zealand revealed that the sample from Scorching Bay, Wellington, fits the Chilean ITS-RFLP patterns. The population from Blockhouse Bay, Auckland, appears to correspond to another species.

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Authors and Affiliations

  1. Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Chile

    Arturo Candia, Mariela A. González & Patricia Gómez

  2. Departamento de Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla, 2407, Concepción, Chile

    Rolando Montoya

  3. Museum of New Zealand Te Papa Tongarewa, PO Box 467, Wellington, New Zealand

    Wendy Nelson

Authors
  1. Arturo Candia
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  2. Mariela A. González
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  3. Rolando Montoya
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  4. Patricia Gómez
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  5. Wendy Nelson
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Candia, A., González, M.A., Montoya, R. et al. Comparison of ITS RFLP patterns of Gracilaria (Rhodophyceae, Gracilariales) populations from Chile and New Zealand and an examination of interfertility of Chilean morphotypes. Journal of Applied Phycology 11, 185–193 (1999). https://doi.org/10.1023/A:1008094009045

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