Abstract
Sixteen species of unialgal samples of dinoflagellate, either wild or cultured, were tested for production of diarrhetic shellfish toxins such as okadaic acid (OA), dinophysistoxin-1 (DTX1), and pectenotoxins (PTXs). Determination of micro-quantities of the toxins was facilitated by fluorometry and UV HPLC. Seven Dinophysis species were confirmed to produce either OA or DTX1, or both. Toxin content and composition varied regionally and seasonally. Intraspecies variation was also observed among cultured strains of Prorocentrum lima. PTX2 was the only toxin detected among PTX family, and D. fortii was the only species to contain this toxin.
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References
Campos MJ, Fraga S, Marino J, Sanchez FJ (1982) Red tide monitoring programme in NW Spain, Report of 1977–1981. International council for the exploration of the sea report 1977–1981, 1–8.
Dahl E, Yndestad H (1985) Diarrhetic shellfish poisoning (DSP) in Norway in the autumn 1984 related to occurrence of Dinophysis spp. In: Anderson D, White AW, Baden DG (eds), Toxic Dinoflagellates. Elsevier, New York, 495–500.
Hamano Y, Kinoshita Y, Yasumoto T (1985) Sukling mice assay for diarrhetic shellfish toxins. In: Anderson D, White AW, Baden DG (eds), Toxic Dinoflagellates. Elsevier, New York, 383–388.
Kat M (1979) The occurrence of Prorocentrum species and coincidental gastrointestinal illness of mussel consummers. In: Taylor DL, Seliger HH (eds), Toxic Dinoflagellates. Elsevier/North Holland, Amsterdam, 215–222.
Krogh P, Edler L, Graneli E, Nyman U (1979) Outbreak of diarrhetic shellfish poisoning on the west coast of Sweden. In: Anderson D, White AW, Baden DG (eds), Toxic Dinoflagellates, Elsevier, New York, 501–503.
Lassus P, Bandouil M, Truquet I, Truquet P, Le Baut C, Pierre JM (1985) Dinophysis acuminata distribution and toxicity along the south Brittany coast (France): Correlation with hydrological parameters. In: Anderson D, White AW, Baden DG (eds), Toxic Dinoflagellates. Elsevier, New York, 159–164.
Lee JS, Yanagi T, Kenma R, Yasumoto T (1987) Fluorometric determination of diarrhetic shellfish toxins by high-performance liquid chromatography. Agric. Biol. Chem. 51: 877–881.
Lee JS, Murata M, Yasumoto T (1988a) Analytical methods for determination of diarrhetic shellfish toxins. In: Natori S, Hashimoto K, Ueno Y (eds), Mycotoxins and Phycotoxins 1988. Elsevier, New York, in press.
Lee JS, Tangen K, Dahl E, Hovvgard P, Yasumoto T (1988b) Diarrhetic shellfish toxins in Norwegian mussels. Nippon Suisan Gakkaishi 54: 1953–1957.
Murata M, Sano M, Iwashita T, Naoki H, Yasumoto T (1986) The structure of Pectenotoxin-3, a new constituent of diarrhetic shellfish toxins. Agric. Biol. Chem. 50: 2693–2695.
Murata M, Kumagai M, Lee JS, Yasumoto T (1987) Isolation and structure of Yessotoxin, a novel polyether compound implicated in diarrhetic shellfish poisoning. Tetrahedron Letters 28: 5869–5872.
Terao K, Ito E, Yanagi T, Yasumoto T (1986) Histopathological studies on experimental marine toxin poisoning. I. Ultrastructural changes in the small intestine and liver of suckling mice induced by dinophysistoxin-1 and pectenotoxin-1. Toxicon 24: 1141–1151.
Yasumoto T, Oshima Y, Sugawara W, Fukuyo Y, Oguri H, Igarashi T, Fujita N (1980) Identification of Dinophysis fortii as the causative organism of diarrhetic shellfish poisoning. Nippon Suisan Gakkaishi 46: 1405–1411.
Yasumoto T, Murata M, Oshima Y, Matsumoto GK, Clardy J (1984) Diarrhetic shellfish poisoning. In: Ragelis EP (ed.), American Chemical Society, Washington, 207–214.
Yasomoto T, Murata M, Oshima Y, Sano M, Matsumoto GK, Clardy J (1985) Diarrhetic shellfish toxins. Tetrahedron 41: 1019–1025.
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Lee, JS., Igarashi, T., Fraga, S. et al. Determination of diarrhetic shellfish toxins in various dinoflagellate species. J Appl Phycol 1, 147–152 (1989). https://doi.org/10.1007/BF00003877
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DOI: https://doi.org/10.1007/BF00003877