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Detection of genetic relationships among fourArtemia species using randomly amplified polymorphic DNA (RAPD)

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International Journal of Salt Lake Research

Abstract

We have applied the technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among four species of brine shrimp:Artemia franciscana, A. urmiana, A. sinica, andA. parthenogenetica. Seventy ten-base synthetic oligonucleotides were used to amplify a total of 458 distinct fragments. DNA polymorphisms were found in all the species examined; the highest percentage of polymorphic bands was found inA. parthenogenetica, with 28.8 per cent. Each species was scored for the presence or absence of every amplification product and the data entered into a binary data matrix. Cluster analysis was then performed to create a dendrogram using UPGMA by the NTSYS program. There are significant differences between bisexual species and parthenogenetic populations.A. parthenogenetica provided 94 specific molecular markers, while bisexual species gave 27 specific molecular markers.A. sinica is a species distinct from the other Old World bisexual species.

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Authors and Affiliations

  1. Department of Biology, Nankai University, 300071, Tianjin, PR China

    Yi Sun, Yi-Cheng Zhong, Wen-Qin Song, Run-Sheng Zhang & Rui-Yang Chen

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  1. Yi Sun
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  2. Yi-Cheng Zhong
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  3. Wen-Qin Song
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  4. Run-Sheng Zhang
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  5. Rui-Yang Chen
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The project supported by National Natural Science Foundation of China. Grant nunber: 39570091.

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Sun, Y., Zhong, YC., Song, WQ. et al. Detection of genetic relationships among fourArtemia species using randomly amplified polymorphic DNA (RAPD). International Journal of Salt Lake Research 8, 139–147 (1999). https://doi.org/10.1007/BF02442127

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