Abstract
We have applied the technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among four species of brine shrimp: Artemia franciscana, A. urmiana, A. sinica, and A. parthenogenetica. Seventy ten-base synthetic oligonucleotides were used to amplify a total of 458 distinct fragments. DNA polymorphisms were found in all the species examined; the highest percentage of polymorphic bands was found in A. parthenogenetica, with 28.8 per cent. Each species was scored for the presence or absence of every amplification product and the data entered into a binary data matrix. Cluster analysis was then performed to create a dendrogram using UPGMA by the NTSYS program. There are significant differences between bisexual species and parthenogenetic populations. A. parthenogenetica provided 94 specific molecular markers, while bisexual species gave 27 specific molecular markers. A. sinica is a species distinct from the other Old World bisexual species.
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Abatzopoulos, T.J., Zhang, B. and Sorgeloos, P. 1998. Artemia tibetiana: preliminary characterization of a new Artemia species found in Tibet (People's Republic of China). International study on Artemia. LIX. International Journal of Salt Lake Research 7: 41–44.
Abreu-Grobois, F.A. 1987. A review of the genetics of Artemia. In: P. Sorgeloos, D.A. Bengtson, W. Decleir, and E. Jaspers (eds.) Artemia Research and Its Applications, Vol. 1, pp. 61–69. Universa Press, Wetteren, Belgium.
Abreu-Grobois, F.A. and Beardmore, J.A. 1982. Genetic differentiation and speciation in the brine shrimp Artemia. In: C. Barigozzi (eds.) Mechanisms of Speciation, pp. 345–376.Alan R. Liss, New York.
Badaracco, G., Baratelli, L., Ginelli, E., Meneveri, R., Plevani, P., Valsasnini, P. and Barigozzi, C. 1987. Variation in repetitive DNA and heterochromatin in the genus Artemia. Chromosoma 95: 71–75.
Badaracco, G., Bellorini, M. and Landsberger, N. 1995. Phylogenetic study of bisexual Artemia using random amplified polymorphic DNA. Journal of Molecular Evolution 41: 150–154.
Badaracco, G., Tubiello, G., Benfante, R., Cotelli, F., Maiorano, D. and Landsberger, N. 1991. Highly repetitive DNA sequence in parthenogenetic Artemia. Journal of Molecular Evolution 32: 31–36.
Barigozzi, C. 1989. Genus Artemia: problems of systematics. In: G. Persoone, P. Sorgeloos, O. Roels and E. Jaspers (eds.) The Brine Shrimp Artemia, Vol. 1, pp. 147–153. Universa Press, Wetteren, Belgium.
Barigozzi, C., Badaracco, G., Plevani, P., Baratelli, L., Profeta, S., Ginelli, E. and Meneveri, R. 1984. Heterochromatin in the genus Artemia. Chromosoma 90: 332–337.
Bowen, S.T., Davis, M.L., Fenster, S.R. and Lindwall, G.A. 1980. Sibling species of Artemia. In: G. Persoone, P. Sorgeloos, O. Roels and E. Jaspers (eds.) The Brine Shrimp Artemia, Vol. 1, pp. 155–167. Universa Press, Wetteren, Belgium.
Cai, H.J. and Hou, L. 1991. A study on karyotypes of Artemia from Liaoning province, China. Natural Science Journal of Liaoning Normal University 14(10): 53–59 [in Chinese].
Cai, Y. 1989. A redescription of the brine shrimp (Artemia sinica). The Wasmann Journal of Biology 47(1–2): 105–110.
Cruces, J., Wonenburger, M.L.G., Diaz-Guerra, M., Sebastiàn, J. and Renart, J. 1986. Satellite DNA in the crustacean Artemia. Gene 44: 341–345.
Garcia, D.K. and Benzie, J.A.H. 1995. RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs. Aquaculture 130: 137–144.
Garcia, D.K., Faggart, M.A., Rhoades, L. and Alcivar-Warren, A.A. 1994. Genetic diversity of cultured Penaeus vannamei shrimp using three molecular genetic techniques. Molecular Marine Biology and Biotechnology 3(5): 270–280.
Günther, R.T. 1890. Crustacea. In: R.T. Günther (ed.) Contributions to the Natural History of Lake Urmia, N. W. Persia and Its Neighborhood. Journal of Linnaeus Society (Zoology) 27: 394–398.
Hou, L., Cai, H.J. and Zou, X.Y. 1993. A study on isozymes of ten Artemia strains from China. Atca Zoology Sinica 39(1): 30–37 [in Chinese].
Hou, L., Cai, H.J., Zou, X.Y. and Yang, G. 1997. Expression of isozyme genes and taxonomic status of bisexual Artemia from China. Acta Zoology Sinica 43(2): 184–191 [in Chinese].
Kellogg, V.A. 1906. A new Artemia and its life conditions. Science 24: 594–596.
Landsberger, N., Cancelli, S., Carettoni, D., Barigozzi, C. and Badaracco, G. 1992. Nucleotide variation and molecular structure of the heterochromatic repetitive Alu I DNA in the brine shrimp Artemia franciscana. Journal of Molecular Evolution 35: 486–491.
Lenz, P. 1980. Ecology of an alkali-adapted variety of Artemia from Mono lake, California, USA. In: G. Persoone, P. Sorgeloos, D. Roels and E. Jaspers (eds.) The Brine Shrimp Artemia, Vol. 3. Ecology, Culturing, Use in Aquaculture, pp. 79–96. Universa Press, Wetteren, Belgium.
Orozco-Castillo, C., Chalmers, K.J., Waugh, R. and Powel, W. 1994. Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87: 934–940.
Perez, M.L., Valverde, J.R., Batuecas, B., Amat, F., Marco, R. and Garrese, R. 1994. Speciation in the Artemia genus: Mitochondrial DNA analysis of bisexual and parthenogenetic brine shrimp. Journal of Molecular Evolution 38: 156–168.
Piccinelli, M. and Prosdocimi, T. 1968. Descrizione tassonomica delle due species Artemia salina L. e Artemia persimilis N. Sp. Inst. Lomb. (Rend. Sci) B, 102: 113–118.
Pilla, E.J.S. and Beardmore, J.A. 1994. Genetic and morphometric differentiation in OLD WORLD bisexual species of Artemia (the brine shrimp). Heredity 73: 47–56.
Rowland, L.J. and Levi, A. 1994. RAPD-based genetic linkage map of blueberry derived from across between diploid species (Vaccinium darrowi and V. elliottii). Theor. Appl. Genet. 87: 863–868.
Scheepers, D., Eloy, M.C. and Briquet, M. 1997. Use of RAPD patterns for clone verification and in studying provenance relationships in Norway Spruce (Picea abies). Theor. Appl. Genet. 94: 480–485.
Sorgeloos, P. 1991. Clarification with regard to the taxonomic status of bisexual Artemia from Asia. Larviculture & Artemia newsletter 19: 45–46.
Triantaphyllidis, G.V., Griel, G.R.T., Abatzopoulos, T.J., Thomas, K.M., Peleman, J., Beardmore, J.A. and Sorgeloos, P. 1997. International study on Artemia. LVII. Morphological and molecular characters suggest conspecificity of all bisexual European and North African Artemia populations. Marine Biology 129: 477–478.
Welsh, J. and McClelland, M. 1990. Fingerprinting genomes using PCR with arbitrarily primers. Nucleic Acids Research 18: 7213–7218.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Scott, V.T. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
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Sun, Y., Zhong, YC., Song, WQ. et al. Detection of genetic relationships among four Artemia species using randomly amplified polymorphic DNA (RAPD). International Journal of Salt Lake Research 8, 139–147 (1999). https://doi.org/10.1023/A:1009039815660
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DOI: https://doi.org/10.1023/A:1009039815660