Abstract
Exogenous applications of auxin to in vitro grown carnation ovaries resulted in an increase in dry mass and a decrease in the levels of endogenous cytokinins within the ovaries. Untreated ovaries showed no significant increase in dry mass. There was however, an increase in endogenous cytokinins over the same period. When 14C-BA was applied to ovaries both with and without exogenous auxin the pattern of growth and cytokinin changes followed a similar trend. Although the BA-metabolites were similar in both treatments, degradative metabolism of the cytokinin was faster and the increase in ovary dry mass greater when auxin was included in the treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Choveaux, NA and VanStaden, J (1981) The effect of l-napthaleneacetic acid on the endogenous cytokinin content of aseptically cultured bark segments of Salix babylonica. Plant and Cell Physiol. 22: 1207–1214
Cook, EL (1986) The senescence of the cut carnation (Dianthus caryophyllus L. cv. White Sim) flower. PhD Thesis, University of Natal, Piemetermaritzburg, South Africa
Cook, EL and VanStaden, J (1983) Senescence of cut carnation flowers. Ovary development and CO2 fixation. Plant Growth Regul. 1: 221–232
Dimalla, GG and VanStaden, J (1980) The effect of silver thiosulphate preservative on the physiology of cut carnations. 1. Influence on longevity and carbohydrate status. Z. Pflanzenphysiol. 99: 9–17.
Dyson, WH, Fox, JE and McChesney, JD (1972) Short-term metabolism of urea and purine cytokinins. Plant Physiol. 49: 506–514
Featonby-Smith, BC and VanStaden, J (1987) Cytokinins in cut carnation flowers: I. The complex in ovaries. Plant Growth Regul. 5: 15–23
Forsyth, C and VanStaden, J (1986) The metabolism and cell division activity of adenine derivatives in soybean callus. J. Plant Physiol. 124: 275–287
Heide, OM (1965) Interaction of temperature, auxins and kinetins in the regeneration ability of Begonia leaf cutting. Physiol. Plant 18: 891–920
Inoue, M, Maeda, E, Yoshida, R and Oritani, I (1979) On the occurrence of a high content of cytokinins in rice callus tissue. Plant and Cell Physiol. 20: 917–924
Jordan, WR and Skoog, F (1971) Effect of cytokinin on growth and auxin in the coleoptiles of derooted Avena seedlings. Plant Physiol. 48: 97–99
Lee, YH, Mok, MC, Mok, DWS, Griffin, DA and Shaw, G (1985) Cytokinin metabolism in Phaseolus embryos. Plant Physiol 77: 635–641
Miller, CO (1965) Evidence for the natural occurrence of zeatin and derivatives: Compounds from maize which promote cell division. Proc. Natl. Acad. Sci. USA 54: 1052–1058
Mothes, K, Engelbrecht, L and Schütte, HR (1961) Über die Akkumulation von α-Aminoisobuttersäure in Blattgewebe unter dem Einfluss von Kinetin. Physiol. Plant 14: 72–75
Nichols, R (1976) Cell enlargement and sugar accumulation in the gynaecium of the glasshouse carnation (Dianthus caryophyllus L.) induced by ethylene. Planta 130: 47–52
Noor Saleh, A and Hemberg, T (1980) The influence of kinetin on the endogenous content of indoleacetic acid in swelling seeds of Phaseolus, Zea and Pinus and young plants of Phaseolus. Physiol. Plant 50: 99–102
Richmond, AE and Lang, A (1957) Effect of kinetin on the protein content and survival of detached Xanthium leaves. Science 125: 650–651
VanStaden, J (1976) Seasonal changes in the cytokinin content of Ginkgo biloba leaves. Physiol. Plant 38: 1–5
VanStaden, J and Dimalla, GG (1980) The effect of silver thiosulphate preservative on the physiology of cut carnations. II. Influence on endogenous cytokinins. Z. Pflanzenphysiol. 99: 19–26
VanStaden, J, Featonby-Smith, BC, Mayak, S, Spiegelstein, H and Halevy, AH (1987) Cytokinins in cut carnation flowers. II. Relationship between endogenous ethylene and cytokinin levels in the petals. Plant Growth Regul. 5: 75–86
Wickson, M and Thimann, KV (1985) The antagonism of auxin and kinetin in apical dominance. Physiol. Plant 11: 62–74
Witham, FH (1968) Effect of 2,4-dichlorophenoxyacetic acid on the cytokinin requirement of soybean cotyledon and tobacco stem pith callus tissues. Plant Physiol. 43: 1455–1457
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Featonby-Smith, B.C., Van Staden, J. & Cook, E.L. Cytokinins in cut carnation flower III. The influence of indoleacetic acid on growth, cytokinin content and metabolism of 14C-benzyladenine in cultured ovaries. Plant Growth Regul 5, 87–96 (1987). https://doi.org/10.1007/BF00024736
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024736