Abstract
Microsomal membrane vesicles and purified plasma membranevesicles obtained from coleoptiles of maize (Zea mays L.) weresubjected to pH shifts from pH 7.8 to 4.7. In the presence of ATPaseinhibitors such as vanadate, net accumulation of radiolabelled butyricand indole-3-acetic acid (IAA) remained higher than in controls. When 2min after the pH shift, at 4°C, the microsomal vesicles weredenatured the amount of ATP could be determined using theluciferin/luciferase assay. Significantly increased ATP production overcontrol values – no pH-shift or ionophore treatment – wasfound. Therefore, such vesicles might produce ATP for in vitrotransport processes such as auxin efflux.
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Godbolé, R., Gräber, P. & Hertel, R. ATP production after pH shift in membrane vesicles from maize coleoptiles. Plant Growth Regulation 32, 151–155 (2000). https://doi.org/10.1023/A:1010797326939
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DOI: https://doi.org/10.1023/A:1010797326939