Abstract
Chlorocholine chloride (CCC) was sprayed on a potato crop 25 days after sowing (DAS) at 5 day intervals for a total of 7 sprays. Activity of sucrose synthase (SS) in the sucrose cleavage direction was many fold higher than that of acid invertase in all the tissues. The activity of alkaline invertase was negligible. A sharp decline in the starch content of stolons of the CCC-sprayed crop was observed between 60 DAS and 70 DAS. This could divert the carbon towards tubers and thus enhancing its availability for starch synthesis. The CCC-treated crop, in general, had higher SS (cleavage) activity in stem, stolons and tubers. A higher sucrose content in the stem of the CCC-treated crop could be due to the high sucrose phosphate synthase (SPS) activity observed in this plant part. In tubers of CCC-treated crops a higher SS (cleavage) activity along with a high sucrose content in tubers during the active tuber filling stage could lead to better availability of UDP-glucose for its conversion to glucose-1-phosphate, which could enter into the amyloplast leading to higher starch content. High SPS activity in tubers of CCC-treated plants ensures that reducing sugars formed are reconverted efficiently to sucrose. The efficiency of developing tubers from CCC-sprayed plants to convert 14C sucrose fed through stolons into starch was about 2.5 times more than in the control.
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References
apRees T and Morrel S (1990) Carbohydrate metabolism in developing potatoes. American Potato J 67: 835-847
Davis BD (1984) Regulation ofα-amylase activity in bean stem tissues. Plant Physiol 74: 841-845
Dimitrov S and Stoinova E (1992) Effect of some synthetic growth regulators on the quality and quantity of potato yield. Fizilogia na Rasteniyata 18: 35-42
Dyson PW (1965) Effects of gibberellic acid and (2-chloroethyl)-trimethylammonium chloride on potato growth and development. J Sci Food Agric 16: 542-549
Golovko TK, Tabalenkova GN and Markarov AM (1992) Effect of photoperiod and chlorocholine chloride on the photosynthetic activity of potato in relation to tuber formation. In Gazoolmen rasteniv posevakh i prirodnykh fitotsenozakh. Jezisydiokladov rabochgo soveshchaniya 17-19
Kerr PS, Torres WK and Huber SC (1987) Resolution of two molecular forms of sucrose phosphate synthase from maize soybean and spinach leaves. Planta 170: 515-519
Kim S-Y, Kim J-K, Ryu O-H, Hhan B-H and Chae J-C (1991) Effect of plant growth regulator application on the growth, tuber yield and dry matter content of potatoes. Res Rep Rural Dev Adm (Suweon) 33: 108-112
Kumar D and Wareing PF (1974) Studies on tuberization of Solanum andigena II. Growth hormones and tuberization. New Phytol 73: 833-840
Kumari S and Bharti S (1992) Effect of CCC and FAP on photosynthesis in sunflower under simulated drought conditions. Haryana Agric Univ J Res 22: 206-213
McRary WL, Slattery M (1945) The colorimetric determination of fructosan in plant material. J Biol Chem 157: 161-167
Menzel CM (1980) Tuberization in potato at high temperatures: Responses of gibberellin and growth inhibitors. Ann Bot 46: 259-265
Miroshnichenko TA and Manankov MI (1991) Effect of gibberellin and chlorocholine chloride on the pigment complex of onion. Fiziol Biokhim Kult Rast 23: 452-455
Morrell S and apRees T (1986) Sugar metabolism in developing tubers of Solanum tuberosum. Phytochem 25: 1579-1585
Nelson N (1944) A photometric adaptation of Somogyi method for the determination of glucose. J Biol Chem 153: 375-380
Nemchenko VV, Versinin YA and Khalitov AK (1981) Effectiveness of chlorocholine chloride applied to potatoes. Khimiya V Sel'skom Khozyaistve 9: 42-45
Pressey R (1969) Potato sucrose synthetase: Purification, properties and changes in activity associated with maturation. Plant Physiol 44: 759-764
Rabha RK and Dey SC (1993) Evaluation of GA and CCC on growth and yield efficiency of potato (Solanum tuberosum L.). Bulletin of Pure and Applied Sciences 12B: 27-30
Ross HA, Davies HV, Burch LR, Viola R and McRae D (1994) Developmental changes in carbohydrate content and sucrose degrading enzymes in tuberising stolons of potato (Solanum tuberosum). Physiol Plant 90: 748-756
Sharma N (1997) Effect of exogenous growth regulators on carbohydrate metabolism in potato. PhD thesis, Punjab Agricultural University, Ludhiana, India
Sowokinos JR (1971) Relationship of sucrose synthetase cleavage activity to the chemical and physical maturity of norchip and kennebec potatoes. Am Potato J 48: 37-46
Sowokinos JR (1973) Maturation of Solanum tuberosum I. Comparative sucrose and sucrose synthetase levels between several good and poor processing varieties. Am Potato J 50: 234-237
Stahli D, Perissin-Tabert D, Blouet A and Guckert A (1995) Contribution of the wheat (Triticum aestivum L.) flag leaf to grain yield in response to plant growth regulators. Plant Growth Regulation 16: 293-297
Van Handel E (1968) Direct micro determination of sucrose. Anal Biochem 22: 280-283
Yadav AC, Mangal JL, Singh A and Singh TP (1992) Induction of salt tolerance in potato. Haryana Agric Univ J Res 22: 76-80
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Sharma, N., Kaur, N. & Gupta, A.K. Effect of chlorocholine chloride sprays on the carbohydrate composition and activities of sucrose metabolising enzymes in potato (Solanum tuberosum L.). Plant Growth Regulation 26, 97–103 (1998). https://doi.org/10.1023/A:1006087729077
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DOI: https://doi.org/10.1023/A:1006087729077