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2-Methylisoborneol disposition in three strains of catfish: absence of biotransformation

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Abstract

2-Methylisoborneol (MIB) and structurally related terpenoid compounds are responsible for millions of dollars of lost revenue to catfish farmers. In an attempt to determine enzymatic pathways of biotransformation and elimination of MIB, the in vitro metabolism of MIB was examined in the Ulvade strain of channel catfish (Ictalurus punctatus). Although cytochrome P450 (CYP) activities were observed and correlated with expression of specific isoforms (i.e. steroid hydroxylation and CYP3A expression), no metabolites of MIB were observed. To determine whether extrahepatic biotransformation may be occurring the in vivo metabolism and disposition of 14C-MIB was examined in Uvalde, USDA-103 channel catfish, and a channel catfish X blue catfish (Ictalurus furcatus) hybrid species. Confirming in vitro hepatic studies, no metabolites were observed in plasma from animals treated with an intra-arterial dose of 14C-MIB. 14C-MIB elimination was predicted using a two compartment model in each strain of fish. There was no significant difference in terminal half-lives between strains but possible differences in total body clearance and apparent volumes of distribution which may be related to higher lipid content in the hybrids. Results of these studies indicate biotransformation has no involvement in MIB elimination and that other physiological processes may play a more significant role in MIB disposition within Ictalurid fish species.

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Authors and Affiliations

  1. Department of Environmental Sciences, University of California, Riverside, Riverside, CA , 92521, USA

    Daniel Schlenk

  2. Department of Pharmacology, Environmental Toxicology Program, School of Pharmacy, University of Mississippi, University, MS , 38677, USA

    Bryan DeBusk

  3. Eli Lilly and Company, Indianapolis, IN , 46140, USA

    Everett J. Perkins

Authors
  1. Daniel Schlenk
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  2. Bryan DeBusk
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  3. Everett J. Perkins
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Schlenk, D., DeBusk, B. & Perkins, E.J. 2-Methylisoborneol disposition in three strains of catfish: absence of biotransformation. Fish Physiology and Biochemistry 23, 225–232 (2000). https://doi.org/10.1023/A:1007834720306

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