Abstract
Two Swedish landlocked (nonanadromous) salmon (Salmo salar) stocks were investigated with the aim of characterising their egg fatty acid (FA) and carotenoid profiles. Fish from one of the stocks were also cultured over the past few decades as part of the Swedish program for genetic preservation, allowing a comparison between the eggs from females on a diet based on lipids of limnic (natural food chain) origin and eggs from females fed an artificial diet of marine origin. No significant differences in the FA profile of the phospholipid (PL) or triacylglycerol (TAG) fraction were found between the two wild stocks. The content of EPA (20:5n-3, eicosapentaenoic acid) in PL fraction was significantly higher in eggs from cultured females (13.0%) compared with eggs from both wild stocks (5.7 and 6.4%). Further, in PL fraction, AA (20:4n-6, arachidonic acid) levels in these eggs were significantly lower (2.4% versus 6.7 and 6.2%). The AA content of the TAG fraction differed greatly between wild (4.4 and 4.9%) and cultured (1.2%) eggs, whereas this fraction showed almost no corresponding difference in EPA content. The level of DHA (22:6n-3, docosahexaenoic acid) did not differ between the two wild stocks or between wild and cultured fish. This was in spite of widely different levels of DHA in the diet. The composition of carotenoids was altered in the cultured eggs which had a higher proportion and higher content (1.16 μ g egg−1) of astaxanthin than the wild eggs (0.56 and 0.62 μg egg−1, respectively). Hatching success varied markedly between wild (>95%) and cultured fish (40–75%). We conclude that changes in the lipid source in the diet of female salmon during gonadal maturation will alter the egg fatty acid composition with an increased risk of disturbances in embryonic development as a consequence. Further, the lack of any difference between wild and cultured females in terms of their egg DHA content indicates that there is a strong genetic influence on levels of this fatty acid in salmon eggs.
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References
Ackman, R.G. and Takeuchi, T. 1986. Comparison of fatty acids and lipids of smolting hatchery-fed and wild Atlantic salmon Salmo salar. Lipids 21: 117–120.
Almer, B. 1979. Vänerprojektet 1972–1977, Fish Report. Information, Sötvattenslaboratoriet, Drottningholm, Stockholm, Sweden.
Bell, J.G., Tocher, D.R., Farndale, B.M., Cox, D.I., McKinney, R.W. and Sargent, J.R. 1997. The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation. Lipids 32: 515–525.
Castell, J.D., Boston, L.D., Miller; R.J. and Kenchington, T. 1995. The potential identification of the geographic origin of lobster eggs from various wild stocks based on fatty acid composition. Can. J. Fish. Aquat. Sci. 52: 1135–1140.
Cowey, C.B., Bell, J.G., Knox, D., Fraser, A. and Youngson, A. 1985. Lipids and antioxidant systems in developing eggs of salmon (Salmo salar). Lipids 20: 567–572.
Evans, R.P., Parrish, C.C., Brown, J.A. and Davis, P.J. 1996. Biochemical composition of eggs from repeat and first-time spawning captive Atlantic halibut (Hippoglossus hippoglossus). Aquaculture 139: 139–149.
Hara, A. and Radin, N.S. 1978. Lipid extraction of tissues with a low toxicity solvent. Anal. Biochem. 90: 420–426.
Hazel, J.R. 1989. Cold adaptation in ectotherms: regulation of membrane function and cellular metabolism. In: Advances in Comparative and Environmental Physiology. Vol. 4, pp. 1–50. Animal Adaptation to Cold. Edited by L.C.H. Wang, Springer-Verlag, Berlin.
Hazel, J.R. and Williams, E.E. 1990. The role of alterations in membrane lipid composition in enabling physiological adaptation of organisms to their physical environment. Progr. Lipid Res. 29: 167–227.
Henderson, R.J. and Tocher, D.R. 1987. The lipid composition and biochemistry of freshwater fish. Progr. Lipid Res. 36: 281–347.
Kehrer, J.P. 1993. Free radicals as mediators of tissue injury and disease. Crit. Rev. Toxicol. 32: 21–48.
Kurashige, M.E., Okimasu, M., Inoue, M. and Utsumi, K. 1990. Inhibition of oxidative injury of biological membranes by astaxanthin. Physiol. Chem. Physics Med. NMR 22: 27–38.
Larsson, P-O. 1980. Smolt rearing and the Baltic salmon fishery. In: Salmon Ranching. pp. 157–186. Edited by J. Thorpe. Academic Press, London.
Linko, R.R., Rajasilta, M. and Hiltunen, R. 1992. Comparison of lipid and fatty acid composition in vendace (Coregonus albula L.) and available plankton feed. Comp. Biochem. Physiol. 103A: 205–212.
Mourente, G. and Odriozola, J.M. 1990. Effect of broodstock diets on total lipids and fatty acid composition of larvae of gilthead sea bream (Sparus aurata L) during yolksac stage. Fish Physiol. Biochem. 2: 103–110.
Mustafa, T. and Srivastava, K.C. 1989. Prostaglandins (eicosanoids) and their role in ectothermic organisms. Adv. Comp. Environ. Physiol. 5: 157–207.
Nilsson, P.-A. 1979. Food and habitat of the fish community of the offshore region of Lake Vänern, Sweden. Institute of Freshwater Research, Drottningholm, Report no. 58.
Nishigaki, I., Dmitrovski, A.A., Miki, W. and Yagi, K. 1994. Suppressive effect of astaxanthin on lipid peroxidation induced in rats J. Clin. Biochem. Nutr. 16: 161–166.
Olsen, R.E. and Ringø, E. 1992. Lipids of Arctic charr, Salvelinus alpinus (L) II. Influence of dietary fatty acids on the elongation and desaturation of linoleic and linolenic acid. Fish Physiol. Biochem. 9: 393–399.
Olsen, R.E. and Henderson, R.J. 1997. Muscle fatty acid composition and oxidative stress indices of Arctic charr, Salvelinus alpinus (L.) in relation to dietary polyunsaturated fatty acid levels and temperature. Aquacult. Nutr. 3: 227–238.
Pettersson, A. and Lignell, Å. 1998a. Low astaxanthin levels in Baltic salmon exhibiting the M74 syndrome. In: Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea. Edited by G. McDonald, J.D. Fitzsimmon and D.C. Honeyfield. Am. Fish. Soc. Symp. 21: 26–30.
Pettersson, A. and Lignell, Å. 1998 b. Astaxanthin deficiency in eggs and fry of Baltic salmon (Salmo salar) with the M74 syndrome. Ambio 28: 43–47.
Pickova, J., Dutta, P., Larsson, P.-O. and Kiessling, A. 1997. Early embryonic cleavage pattern, hatching success and egg-lipid fatty acid composition: Comparison between two cod stocks (Gadus morhua). Can. J. Fish. Aquat. Sci. 54: 2410–2416.
Pickova, J., Kiessling, A., Pettersson, A. and Dutta, P.C. 1998. Comparison of fatty acid composition and astaxanthin content in healthy and by M74 affected salmon eggs from three Swedish river stocks. Comp. Biochem. Physiol. 120B: 265–271.
Rottiers, V.D. 1993. Elemental composition of a migratory and a land-locked strain of Atlantic salmon Salmo salar. Comp. Biochem. Physiol. 104A: 93–100.
Sargent, J.R. 1995. Origins and functions of egg lipids: Nutritional implications In: Brood Stock Management and Egg and Larval Quality pp. 353–372. Edited by N.R. Bromage and R.J. Roberts Blackwell Science, Cambridge, U.K.
Sargent, J.R., Bell, J.G., Bell, M.V., Henderson, R.J. and Tocher, D.R. 1995. Requirement criteria for essential fatty acids. J. Appl. Ichthyol. 11: 183–198.
Sargent, J.R., Mc Evoy, L.A. and Bell, J.G. 1997. Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds. Aquaculture 155: 117–127.
Svärdsson, G. 1945. Chromosome studies on Salmonidae. Rep. Inst. Freshw. Res. Drottningholm 23: 1–151.
Watanabe, T. 1982. Lipid nutrition in fish. Comp. Biochem. Physiol. 73 B: 3–15.
Wiegand, M.D. 1996. Composition, accumulation and utilization of yolk lipids in teleost fish. Rev. Fish Biol. Fish. 6: 259–286.
Wiegand, M.D. and Idler, D.R. 1985. Ovarian neutral lipid fatty acid composition varies with state of ovarian growth in landlocked Atlantic salmon. Can. J. Zool. 63: 2775–2777.
Youngson, A.F, Mitchell, A.I., Noack, P.T. and Laird, L.M. 1997. Carotenoid pigment profiles distinguish anadromous and nonanadromous brown trout (Salmo trutta). Can. J. Fish. Aquat. Sci. 54: 1064–1066.
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Pickova, J., Kiessling, A., Pettersson, A. et al. Fatty acid and carotenoid composition of eggs from two nonanadromous Atlantic salmon stocks of cultured and wild origin. Fish Physiology and Biochemistry 21, 147–156 (1999). https://doi.org/10.1023/A:1007860908911
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DOI: https://doi.org/10.1023/A:1007860908911