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Effects of carbamazepine on nerve activity and transmitter release in neuroblastoma-glioma hybrid cells and the frog neuromuscular junction

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Abstract

Effects of the antiepileptic drug carbamazepine on nerve action potential and transmitter release in mouse neuroblastoma-glioma hybrid cells (NG108-15) and the frog neuromuscular junction were studied. Carbamazepine within a concentration range of 0.1–0.5 mmol/L reduced the peak height of the action potential of the NG108-15 cells, whereas the membrane potential and membrane resistance were unaffected. Voltage clamp revealed that the decrease in the action potential was due to the blockage of the Na+, delayed K+ and transient Ca2+ currents. Carbamazepine did not affect Ca2+-activated and A type K+ currents and long-lasting Ca2+ current. In the frog neuromuscular junction, carbamazepine decreased the mean quantal content by a parallel shift in the frequency augmentation–potentiation (FAP) relation. It is concluded that carbamazepine blocks the voltage-dependent Na+, delayed K+, and transient Ca2+ currents and quantal transmitter release through a decrease of nerve excitation.

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References

  • Alderdice MT, Trommer BA. Differential effects of the anti-convulsants phenobarbital, ethosuximide and carbamazepine on neuromuscular transmission. J Pharmacol Exp Ther. 1980;215:92–6.

    PubMed  Google Scholar 

  • Aronson JK. Potassium channels in nervous tissue. Biochem Pharmacol. 1992;43:11–4.

    Article  PubMed  Google Scholar 

  • Backus KH, Pflimlin P, Trube G. Action of diazepam on the voltage-dependent Na+ current: comparison with the effects of phenytoin, carbamazepine, lidocaine and flumazenil. Brain Res. 1991;548:41–9.

    Article  PubMed  Google Scholar 

  • Enomoto K, Higashida H, Maeno T. Effects of toki-shakuyakusan (Tsumura TJ-23) on electrical activity in neuroblastoma cells and frog neuromuscular junctions. Neurosci Res. 1992; 15:81–9.

    Article  PubMed  Google Scholar 

  • Furuya K, Furuya S, Yamagishi S. Developmental time courses of Na and Ca spikes in neuroblastoma × glioma hybrid cells. Dev Brain Res. 1983;11:229–34.

    Article  Google Scholar 

  • Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981;391:85–100.

    Google Scholar 

  • Hille B. Potassium channels and chloride channels. In: Ionic channels of excitable membranes. Sunderland MS: Sinauer Associates; 1984:113.

    Google Scholar 

  • Kasai H, Neher E. Dihydropyridine-sensitive and ω-conotoxin-sensitive calcium channels in a mammalian neuroblastomaglioma cell line. J Physiol. 1992;448:161–88.

    PubMed  Google Scholar 

  • Kito M, Maehara M, Watanabe K. Antiepileptic drugs-calcium current interaction in cultured human neuroblastoma cells. Seizure. 1994;3:141–9.

    PubMed  Google Scholar 

  • Lancaster JM, Davies JA. Carbamazepine inhibits NMDA-induced depolarizations in cortical wedges prepared from DBA/2 mice. Experientia. 1992;48:751–3.

    PubMed  Google Scholar 

  • Lang DG, Wang CM, Cooper BR. Lamotrigine, phenytoin and carbamazepine interactions on the sodium current present in N4TG1 mouse neuroblastoma cells. J Pharmacol Exp Ther. 1993;266:829–35.

    PubMed  Google Scholar 

  • Macdonald RL, Kelly KM. Antiepileptic drug mechanisms of action. Epilepsia. 1995;36:S2–12.

    Google Scholar 

  • Maeno T, Enomoto K. Kinetic analysis of transmitter release in neuromuscular transmission. Adv Biophys. 1988;24:93–122.

    Article  PubMed  Google Scholar 

  • Morselli PL. Carbamazepin: absorption, distribution and excretion. In: Levy R, Mattson R, Meldrum B, Penry JK, Dreifuss FE, eds. Antiepileptic drugs. New York: Raven Press; 1989:473–90.

    Google Scholar 

  • Nowycky MC, Fox AP, Tsien RW. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985;404:440–3.

    Google Scholar 

  • Olpe H, Kolb CN, Hausdorf A, Haas HL. 4-Aminopyridine and barium chloride attenuate the anti-epileptic effect of carbamazepine in hippocampal slices. Experientia. 1991;47:254–7.

    PubMed  Google Scholar 

  • Robbins J, Sim JA. A transient outward current in NG108-15 neuroblastoma-glioma hybrid cells. Pflugers Arch. 1990; 416:130–7.

    PubMed  Google Scholar 

  • Schirrmacher K, Mayer A, Walden J, Dusing R, Bingmann D. Effects of carbamazepine on action potentials and calcium currents in rat spinal ganglion cells in vitro. Neuropsychobiology. 1993;27:176–9.

    PubMed  Google Scholar 

  • Twombly DA, Hermank MD, Kye CH, Narahashi T. Ethanol effects on two types of voltage-activated calcium channels. J Pharmacol Exp Ther. 1990;254:1029–37.

    PubMed  Google Scholar 

  • Walden J, Grunze H, Mayer A et al. Calcium-antagonistic effects of carbamazepine in epilepsies and affective psychoses. Neuropsychobiology. 1993;27:171–5.

    PubMed  Google Scholar 

  • Willow M, Gonoi T, Catterall WA. Voltage clamp analysis of the inhibitory actions of diphenylhydantoin and carbamazepine on voltage-sensitive sodium channels in neuroblastoma cells. Mol Pharmacol. 1985;27:549–58.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Neurosurgery, Shimane Medical University, Izumo, Shimane, Japan

    Y. Matsumoto & K. Moritake

  2. Department of Physiology, Shimane Medical University, Izumo, Shimane, Japan

    K. Enomoto & T. Maeno

Authors
  1. Y. Matsumoto
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  2. K. Enomoto
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  3. K. Moritake
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  4. T. Maeno
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Matsumoto, Y., Enomoto, K., Moritake, K. et al. Effects of carbamazepine on nerve activity and transmitter release in neuroblastoma-glioma hybrid cells and the frog neuromuscular junction. Cell Biol Toxicol 14, 191–198 (1998). https://doi.org/10.1023/A:1007410509772

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  • DOI: https://doi.org/10.1023/A:1007410509772

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