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Detection of guanylate cyclases A and B stimulated by natriuretic peptides in gastrointestinal tract of rat

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Summary

The ultracytochemical localization of membrane-bound guanylate cyclases A and B has been studied after stimulation with atrial natriuretic peptide, C-type natriuretic peptide and brain natriuretic peptide in the gastrointestinal tract of rat. The two isoforms are stimulated differently by the three peptides. The results showed that the atrial and C-type natriuretic peptides stimulated guanylate cyclase activity, whereas the brain peptide seemed not to activate enough of the enzyme to detect. The guanylate cyclase activity had a wider distribution in stomach and small intestine than in large intestine; nevertheless, the reaction product of guanylate cyclase A activity had a wider localization in the stomach, whereas the reaction product of guanylate cyclase B activity had a wider distribution in the small intestine. In the small and large intestine, we detected mostly similar localizations of guanylate cyclase activity irrespective of the peptide used; in the stomach the reaction products of guanylate cyclase A and B were detected in different cell types or in different sites of the same cell. In all the gastrointestinal tract, guanylate cyclase activity was detected mainly in three types of cells: exocrine and endocrine cells; undifferentiated and mature epithelial cells; and smooth muscle cells. These localizations of guanylate cyclase activity suggest its role in regulating glandular secretion, cellular proliferation and muscular activity.

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  1. Section of Anatomy, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Cas. Post. 81, Succ. 3, 06126, Perugia, Italy

    Maria Grazia Rambotti, Ileana Giambanco & Antonio Spreca

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  1. Maria Grazia Rambotti
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  2. Ileana Giambanco
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  3. Antonio Spreca
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Rambotti, M.G., Giambanco, I. & Spreca, A. Detection of guanylate cyclases A and B stimulated by natriuretic peptides in gastrointestinal tract of rat. J Mol Hist 29, 117–126 (1997). https://doi.org/10.1023/A:1026429221065

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