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An immunological study on chromogranin A and B in human endocrine and nervous tissues

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Summary

Antisera were raised against synthetic peptides derived from the primary amino acid sequence of human chromogranin B. These antisera recognized in one- and two-dimensional immunoblotting a component previously designated as chromogranin B. In human chromaffin granules, the major endogenous processing product of chromogranin B is formed by proteolytic cleavage of the protein near theC-terminus. Immunohistochemical localizations were obtained with antisera against human chromogranins A and B and against a synthetic peptide corresponding to the B sequence. In human tissues, chromogranin B is co-stored with chromogranin A in the adrenal medulla, the anterior pituitary, parafollicular cells of the thyroid, in some cells of the endocrine pancreas and in some enterochromaffin cells, whereas only chromogranin A is found in the parathyroid gland and enterochromaffin cells of the gastric corpus mucosa. In the nervous system, no immunostaining was observed for chromogranin A and only a weak one for chromogranin B in some cells of the spinal cord. However, the Purkinje cells of the cerebellum were strongly positive for chromogranin B.

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Authors and Affiliations

  1. Department of Pathology, University of Innsbruck, Innsbruck, Austria

    K. W. Schmid

  2. Department of Pharmacology, University of Innsbruck, Innsbruck, Austria

    R. Weiler, R. Fischer-Colbrie & H. Winkler

  3. Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, USA

    R. W. Xu & R. Hogue-Angeletti

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  1. K. W. Schmid
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  2. R. Weiler
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  3. R. W. Xu
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  4. R. Hogue-Angeletti
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  5. R. Fischer-Colbrie
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  6. H. Winkler
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Schmid, K.W., Weiler, R., Xu, R.W. et al. An immunological study on chromogranin A and B in human endocrine and nervous tissues. Histochem J 21, 365–372 (1989). https://doi.org/10.1007/BF01798500

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  • DOI: https://doi.org/10.1007/BF01798500

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