biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 39:561-567, 1997 | DOI: 10.1023/A:1000926714622

The effect of polyamines on the development of sugar beet protoplasts

A. Majewska-Sawka1, A. Niklas1, E. Jażdżewska1
1 Institute for Plant Breeding and Acclimatization, Bydgoszcz, Poland

The influence of the exogenous polyamines: putrescine, spermidine and spermine, on the frequency of protoplast divisions for 2 genotypes of sugar beet (Beta vulgaris L.) was analyzed. Protoplasts were cultured by the agarose disk method on Saunders and Doley medium supplemented with either hormones or polyamines, or hormones combined with polyamines. The latter supplement led to a statistically significant increase in plating efficiency. The improvement in division index was caused mainly by spermine.

Keywords: Beta vulgaris; plating efficiency; protoplasts; putrescine; spermidine; spermine
Subjects: Beta vulgaris; development, protoplast; plating efficiency, protoplasts; polyamines, protoplast development; putrescine, protoplast division; spermidine, protoplast division; spermine, protoplast division; sugar beet, protoplast development

Prepublished online: May 1, 1997; Published: December 1, 1997  Show citation

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Majewska-Sawka, A., Niklas, A., & Jażdżewska, E. (1997). The effect of polyamines on the development of sugar beet protoplasts. Biologia plantarum, 39(4), 561-567. doi: 10.1023/A:1000926714622
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